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Submit ReviewKey Information: Janelia Fluor® 635, Tetrazine is a red fluorogenic fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Janelia Fluor® 635, Tetrazine is cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 645 nm and 663 nm, respectively.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Emission Color | Red |
---|---|
λabs | 635 nm |
λem | 652 nm |
Cell Permeable | Yes |
Application | Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry |
Alternative For | Alexa Fluor® 633, Atto 633, CF633 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 701.81 |
公式 | C38H33F2N7O3Si |
储存 | Store at -20°C |
纯度 | ≥90% (HPLC) |
InChI Key | DVDISOMTYSQEME-UHFFFAOYSA-N |
Smiles | C[Si](C1=C/C2=[N+]3CC(C/3)F)(C4=CC(N5CC(C5)F)=CC=C4C(C6=CC(C(NCC7=CC=C(C8=NN=CN=N8)C=C7)=O)=CC=C6C([O-])=O)=C1C=C2)C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 7.02 | 10 |
以下数据基于产品分子量 701.81。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.1 mM | 14.25 mL | 71.24 mL | 142.49 mL |
0.5 mM | 2.85 mL | 14.25 mL | 28.5 mL |
1 mM | 1.42 mL | 7.12 mL | 14.25 mL |
5 mM | 0.28 mL | 1.42 mL | 2.85 mL |
参考文献是支持产品生物活性的出版物。
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关键词: Janelia Fluor® 635, Tetrazine, Janelia Fluor® 635, Tetrazine supplier, JF635, Janelia, Fluor, 635-Tetrazine, fluorescent, dyes, Tetrazine, cell, permeable, super, resolution, flow, cytometry, dSTORM, STED, copper-free, click, chemistry, Click, Reactive, Dyes, 8134, Tocris Bioscience
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