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Submit ReviewJanelia Fluor® 646, Azide
说明： Red dye (Reactive group: azide). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
别名： JF 646
化学名： 2-(3-(Azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)carbamoyl)benzoate
纯度： ≥95% (HPLC)
生物活性 for Janelia Fluor® 646, Azide
Key Information: Janelia Fluor® 646, Azide is a red fluorogenic fluorescent dye, supplied with an azide reactive handle for copper-free click chemistry. Suitable for live cell imaging.
Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two color imaging with Janelia Fluor® 549 SE (Cat. No. 6147). Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, Azide
| Brightness | |
|---|---|
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 640 nm |
| Reactive Group | Azide |
| Reactivity | Click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.19 |
| Application | dSTORM, STED, Confocal Microscopy |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for Janelia Fluor® 646, Azide
| 分子量 | 696.88 |
| 公式 | C37H44N6O6Si |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| InChI Key | FUJDWZJKTJZMHX-UHFFFAOYSA-N |
| Smiles | O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 646, Azide
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMF | 34.84 | 50 |
制备储备液 for Janelia Fluor® 646, Azide
以下数据基于产品分子量 696.88。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 2.87 mL | 14.35 mL | 28.7 mL |
| 2.5 mM | 0.57 mL | 2.87 mL | 5.74 mL |
| 5 mM | 0.29 mL | 1.43 mL | 2.87 mL |
| 25 mM | 0.06 mL | 0.29 mL | 0.57 mL |
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产品说明书 for Janelia Fluor® 646, Azide
参考文献 for Janelia Fluor® 646, Azide
参考文献是支持产品生物活性的出版物。
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 12 244 PMID: 25599551
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 646, Azide, please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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