Janelia Fluor® 646, free acid

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说明: Red dye (Reactive group: free acid). Application: flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
化学名: 1-[7-(1-Azetidinyl)-10-(2,5-dicarboxyphenyl)-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium inner salt
纯度: ≥95% (HPLC)
说明书
引用文献
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生物活性 for Janelia Fluor® 646, free acid

Key Information: Janelia Fluor® 646, free acid is a red fluorogenic fluorescent dye; supplied with a free acid reactive group. Suitable for live cell imaging.

Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) including dSTORM (in both live and fixed cells) and STED. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1. A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.

To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

This product will replace Janelia Fluor® 646, free acid (Cat. No. 6993).

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Usage Guidelines for Janelia Fluor® 646, free acid

This product is provided for use in onward chemistry. Suitable solvents can be used.

Optical Data for Janelia Fluor® 646, free acid

Janelia Fluor<sup>®</sup> 646, free acid Dye Spectra
Emission Color Red
λabs 646 nm
λem 664 nm
Extinction Coefficient (ε) 152,000 M-1cm-1
Quantum Yield (φ) 0.54
Closest Laser line 640 nm
Reactive Group Free acid
Reactivity Primary amines
Cell Permeable Yes
Correction Factor 280 0.19
Application Flow Cytometry, Confocal Microscopy, dSTORM, STED, Live-cell Imaging
Alternative For Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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技术数据 for Janelia Fluor® 646, free acid

分子量 496.64
公式 C29H28N2O4Si
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 1811539-57-7
PubChem ID 140772579
InChI Key YIPCOENULPIGPZ-UHFFFAOYSA-N
Smiles OC(C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Janelia Fluor® 646, free acid

参考文献 for Janelia Fluor® 646, free acid

参考文献是支持产品生物活性的出版物。

Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands and their use in cellular imaging experiments. Methods Mol.Biol. 1663 179 PMID: 28924668

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID: 25599551

Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787


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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including Janelia Fluor® Dyes
  • Fluorescent Probes for Advanced Microscopy and Live Cell Imaging
  • Fluorescent Probes to Support In Vivo, Deep Tissue, and Bioluminescence Imaging
  • Fluorescent Probes and Reagents for Organoids and 3D Cell Culture Imaging
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals
  • Tissue Clearing Kits and Reagents