Janelia Fluor^® 669, NHS ester

Cat. No. 6420 1 次引用提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Far red dye (Reactive group: NHS ester). Application: confocal miocrocopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging

别名： JF 669,NHS

化学名： 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt

纯度： ≥90% (HPLC)

引用文献 (1)

实验方案 (1)

文献 (1)

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for Janelia Fluor^® 669, NHS ester

Key Information: Janelia Fluor^® 669, NHS ester is a red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Excitation and emission maxima (λ) are 669 nm and 682 nm, respectively; quantum yield = 0.37; extinction coefficient = 116,000 M^-1cm^-1; A280 correction factor = 0.043. Retains 97% fluorescence after 30 bleaching cycles.

Please see the protocol for further information on protein/antibody labeling and conjugation.

We also offer Janelia Fluor^® conjugated antibodies and custom conjugation services.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was Janelia Fluor^® 669, SE.

Scientific Data

Confocal Microscopy

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Applications on Tubulin for Janelia Fluor^® 669, NHS ester. Application of Janelia Fluor^® 669, NHS ester dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor^® 669, NHS ester. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data for Janelia Fluor^® 669, NHS ester

Janelia Fluor<sup>®</sup> 669, NHS ester Dye Spectra

Emission Color	Far Red
Brightness
λ_abs	669 nm
λ_em	682 nm
Extinction Coefficient (ε)	116,000 M^-1cm^-1
Quantum Yield (φ)	0.37
Closest Laser line	640 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Cell Permeable	Yes
Correction Factor 280	0.043
Application	Confocal Microscopy, Super Resolution Microscopy (SRM)
Alternative For	Alexa Fluor^® 660, Atto 655, Atto 665, CF660R

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Janelia Fluor^® 669, NHS ester

分子量	693.77
公式	C₃₄H₃₀F₃N₃O₆SSi
储存	Store at -20°C
纯度	≥90% (HPLC)
CAS Number	2127150-20-1
PubChem ID	132470916
InChI Key	QFHKALIQFJQJKE-UHFFFAOYSA-N
Smiles	C[Si](C1=CC(N2CCC2)=CC=C1C(C3=C(F)C(SCC(ON4C(CCC4=O)=O)=O)=C(F)C(F)=C3C([O-])=O)=C5C=C/6)(C)C5=CC6=[N+]7CCC\7

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Janelia Fluor^® 669, NHS ester

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	13.88	20

制备储备液 for Janelia Fluor^® 669, NHS ester

以下数据基于产品分子量 693.77。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.2 mM	7.21 mL	36.03 mL	72.07 mL
1 mM	1.44 mL	7.21 mL	14.41 mL
2 mM	0.72 mL	3.6 mL	7.21 mL
10 mM	0.14 mL	0.72 mL	1.44 mL

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产品说明书 for Janelia Fluor^® 669, NHS ester

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Janelia Fluor^® 669, NHS ester

参考文献是支持产品生物活性的出版物。

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID: 28869757

Grimm et al (2017) General synthetic method for Si-Fluoresceins and Si-Rhodamines. ACS Cent.Sci. 3 975 PMID: 28979939

Grimm et al (2020) A general method to optimize and functionalize red-shifted rhodamine dyes. Nat.Methods 17 815 PMID: 32719532

If you know of a relevant reference for Janelia Fluor^® 669, NHS ester, please let us know.

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1 篇 Janelia Fluor^® 669, NHS ester 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Janelia Fluor^® 669, NHS ester 的部分引用包括：

Hui et al (2021) EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization. Cell 184 6361-6377.e24 PMID: 34875226

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