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说明： Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
化学名： 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
纯度： ≥95% (HPLC)
生物活性 for NADA-green
NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.
Please note that the spectral data is solvent dependant
Optical Data for NADA-green
| Emission Color | Green |
|---|---|
| λabs | 450 nm |
| λem | 555 nm |
| Extinction Coefficient (ε) | 25,000 M-1cm-1 |
| Application | Fluorescence Microscopy |
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技术数据 for NADA-green
| 分子量 | 303.66 |
| 公式 | C9H9N5O5.HCl |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 2253733-11-6 |
| PubChem ID | 137919868 |
| InChI Key | NKFVSANWNYYLLB-PGMHMLKASA-N |
| Smiles | N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for NADA-green
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 30.37 | 100 |
制备储备液 for NADA-green
以下数据基于产品分子量 303.66。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.29 mL | 16.47 mL | 32.93 mL |
| 5 mM | 0.66 mL | 3.29 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.29 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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产品说明书 for NADA-green
参考文献 for NADA-green
参考文献是支持产品生物活性的出版物。
Kuru et al (2012) In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266
Kuru et al (2014) Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 10 33 PMID: 25474031
Ladokhin et al (2002) Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 41 13617 PMID: 12427023
If you know of a relevant reference for NADA-green, please let us know.
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关键词: NADA-green, NADA-green supplier, fluorescent, green, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, Unnatural, Amino, Acids, Fluorescent, Probes, for, Imaging, Bacteria, 6648, Tocris Bioscience
2 篇 NADA-green 的引用文献
引用文献是使用了 Tocris 产品的出版物。 NADA-green 的部分引用包括：
Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906
Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals