Nucleic Acid Dye Green I

Name: Nucleic Acid Dye Green I
Brand: Tocris Bioscience
SKU: 7246

Cat. No. 7246 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Discontinued Product

7246 has been discontinued.

View all Fluorescent DNA Stains products.

说明： High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR

化学名： 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride

纯度： ≥95% (HPLC)

说明书

引用文献

文献 (1)

生物活性技术数据说明书参考文献

生物活性 for Nucleic Acid Dye Green I

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information for Nucleic Acid Dye Green I

Supplied as a 10,000x concentrate in DMSO.
Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data for Nucleic Acid Dye Green I

Emission Color	Green
λ_abs	495 nm
λ_em	520 nm
Application	Real Time PCR, Nucleic Acid Staining, RT-LAMP
Alternative For	SYBR^® Green I

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Nucleic Acid Dye Green I

分子量	545.19
公式	C₃₂H₃₇ClN₄S
储存	Store at -20°C
纯度	≥95% (HPLC)
CAS Number	2225748-05-8
PubChem ID	131742173
InChI Key	IHHRNZWXTFDDLM-UHFFFAOYSA-M
Smiles	CCCN(C(N(C1=C/2C=CC=C1)C3=CC=CC=C3)=CC2=C\C4=[N+](C)C5=CC=CC=C5S4)CCCN(C)C.[Cl-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Nucleic Acid Dye Green I

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Nucleic Acid Dye Green I

参考文献是支持产品生物活性的出版物。

Karlsen et al (1995) SYBR Green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J.Virol.Methods 55 153 PMID: 8576305

Bookout et al (2006) High-throughput real-time quantitative reverse transcription PCR. Curr.Protoc.Mol.Biol. doi: 10.1002/0471142 PMID: 18265376

Lamb et al (2018) Rapid detection of Zika virus in urine samples and infected mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification. Sci.Rep. 8 3803 PMID: 29491389

Lamb et al (2020) Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification PLoS One 15 e0234682 PMID: 32530929

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关键词: Nucleic Acid Dye Green I, Nucleic Acid Dye Green I supplier, High, affinity, double-stranded, DNA, (dsDNA), probe, RT-LAMP, Reverse, Transcription, Loop-Mediated, Isothermal, Amplification, SARS-CoV-2, Zika, RTPCR, RT-PCR, qPCR, q_PCR, Fluorescent, Stains, RNA, Probes, Loop-mediated, and, RT-qPCR, 7246, Tocris Bioscience

篇 Nucleic Acid Dye Green I 的引用文献

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目前没有 Nucleic Acid Dye Green I 的引用文献。