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PA Janelia Fluor® 646, NHS ester

Cat. No. 6150 3 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Red photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
化学名： 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate
纯度： ≥90% (HPLC)
说明书
引用文献 (3)
评论 (2)
实验方案 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for PA Janelia Fluor® 646, NHS ester

Key information: PA Janelia Fluor® 646, NHS ester is a photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.

Application: Suitable for single molecule tracking and super resolution microscopy in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.

Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 549, NHS ester (Cat. No. 6149) to perform two-color sptPALM in live cells with activation at 405nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining. Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 646, SE.

许可信息

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for PA Janelia Fluor® 646, NHS ester

分子量 617.74
公式 C34H31N5O5Si
储存 Store at -20°C
纯度 ≥90% (HPLC)
CAS Number 2093120-32-0
PubChem ID 127021042
InChI Key XVIATAHKOZWKHH-UHFFFAOYSA-N
Smiles O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5[Si](C)(C)C7=CC(N8CCC8)=CC=C47)=C2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for PA Janelia Fluor® 646, NHS ester

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 12.35 20

制备储备液 for PA Janelia Fluor® 646, NHS ester

以下数据基于产品分子量 617.74。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.2 mM 8.09 mL 40.47 mL 80.94 mL
1 mM 1.62 mL 8.09 mL 16.19 mL
2 mM 0.81 mL 4.05 mL 8.09 mL
10 mM 0.16 mL 0.81 mL 1.62 mL

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产品说明书 for PA Janelia Fluor® 646, NHS ester

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for PA Janelia Fluor® 646, NHS ester

参考文献是支持产品生物活性的出版物。

Grimm et al (2016) Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 13 985 PMID: 27776112

Li et al (2016) Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 5 e17056 PMID: 27484239

If you know of a relevant reference for PA Janelia Fluor® 646, NHS ester, please let us know.

按标靶查看相关产品

关键词: PA Janelia Fluor 646, NHS ester, PA Janelia Fluor 646, NHS ester supplier, PA, JF646, Photoactivatable, caged, Janelia, Fluor, 646-NHS, ester, fluorescent, dyes, NHS, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, 646-SE, Dyes, 6150, Tocris Bioscience

3 篇 PA Janelia Fluor® 646, NHS ester 的引用文献

引用文献是使用了 Tocris 产品的出版物。 PA Janelia Fluor® 646, NHS ester 的部分引用包括：

Sheila S et al (2019) Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaging. Methods Mol Biol 2038 239-250 PMID: 31407289

Jeroen et al (2022) Microscopy-based single-cell proteomic profiling reveals heterogeneity in DNA damage response dynamics. Cell Rep Methods 2 100237 PMID: 35784653

Genshaft et al (2021) Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes Nat Commun 12 4995 PMID: 34404785

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BG labelled PA JF646.
By Abigail Walker on 05/21/2024
分析类型： In Vitro
种属： Human
细胞系/组织： HEK 293 cells

We conjugated PA JF656 with the SNAP label BG, and used it for single molecule imaging in HEK 293 cells (both live and fixed). The dye works very well, it is stable, bright, and activates and bleaches well (better than other photoactivatable dyes I have used).We have used it for imaging at 1-10 nM as we found some non-specific binding at concentrations higher than this, but potentially this could be removed with additional wash steps.

review image
PA-JF646 for tracking single recombinant proteins.
By Anonymous on 02/26/2018
分析类型： In Vitro
种属： Other

This product was used to label recombinant proteins and image them with single molecule sensitivity. The degree of labeling achieved was 1.4 (average). The photoactivation efficiency of the dye is excellent and can be used to track a few molecules at a time, for long periods. The dye is highly photostable and can be used to track proteins for ~10-15 seconds under ideal tracking conditions. I attach here an image before and after photoactivation of the dye. Upon photoactivation, single dye molecules conjugated to the protein are visible.

review image

PA Janelia Fluor® 646, NHS ester 实验方案

以下实验方案包含使用 PA Janelia Fluor® 646, NHS ester（目录编号 6150）的更多信息。

该领域的文献

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