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Submit ReviewPhorbol 12,13-dibutyrate
说明： Protein kinase C activator
别名： PDBu
化学名： (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
纯度： ≥97% (HPLC)
生物活性 for Phorbol 12,13-dibutyrate
Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.
For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs
技术数据 for Phorbol 12,13-dibutyrate
| 分子量 | 504.61 |
| 公式 | C28H40O8 |
| 储存 | Store at -20°C |
| 纯度 | ≥97% (HPLC) |
| CAS Number | 37558-16-0 |
| PubChem ID | 37783 |
| InChI Key | BQJRUJTZSGYBEZ-YVQNUNKESA-N |
| Smiles | OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Phorbol 12,13-dibutyrate
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 25.23 | 50 |
制备储备液 for Phorbol 12,13-dibutyrate
以下数据基于产品分子量 504.61。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.96 mL | 19.82 mL | 39.63 mL |
| 2.5 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 5 mM | 0.4 mL | 1.98 mL | 3.96 mL |
| 25 mM | 0.08 mL | 0.4 mL | 0.79 mL |
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产品说明书 for Phorbol 12,13-dibutyrate
参考文献 for Phorbol 12,13-dibutyrate
参考文献是支持产品生物活性的出版物。
Middleton et al (1993) Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 268 15958 PMID: 8393456
Hori et al (1999) Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 19 7262 PMID: 10460232
Sakai et al (2010) Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 46 259 PMID: 21187674
If you know of a relevant reference for Phorbol 12,13-dibutyrate, please let us know.
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关键词: Phorbol 12,13-dibutyrate, Phorbol 12,13-dibutyrate supplier, PDBu, protein, kinases, c, pkc, activators, phorbol, esters, Protein, Kinase, C, Enzyme, Substrates, /, Activators, 4153, Tocris Bioscience
11 篇 Phorbol 12,13-dibutyrate 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Phorbol 12,13-dibutyrate 的部分引用包括：
Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813
Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol 4 591 PMID: 25843049
Sarah C et al (2021) A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice. Med (N Y) 2 243-262.e8 PMID: 33521747
Timothy S et al (2021) Differential Regulation of ATP- and UTP-Evoked Prostaglandin E2 and IL-6 Production from Human Airway Epithelial Cells. J Immunol 207 1275-1287 PMID: 34389624
Yi et al (2012) Constriction of retinal arterioles to endothelin-1: requisite role of rho kinase independent of protein kinase C and L-type calcium channels. Invest Ophthalmol Vis Sci 53 2904-12 PMID: 22427601
Sánchez et al (2014) Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors. Br J Pharmacol 171 5682 PMID: 25091502
Gareth E et al (2022) Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv 8 eabm3471 PMID: 35427166
Oliver T et al (2022) Do more with Less: Improving High Parameter Cytometry Through Overnight Staining. Curr Protoc 2 e589 PMID: 36373983
Nidhi et al (2020) Neonatal-derived IL-17 producing dermal γδ T cells are required to prevent spontaneous atopic dermatitis. Elife 9 PMID: 32065580
Xavier et al (2020) BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11 378 PMID: 32424151
Zoltan et al (2020) A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Rep 32 107968 PMID: 32726631
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PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes.
By
Chintan Koyani on 10/22/2018
分析类型： In Vitro
种属： Other
细胞系/组织： ventricular cardiomyocytes
Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.
