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Ponceau S Staining Solution

Cat. No. 5225 1 次引用 3 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Stain used to detect protein bands after Western blotting
化学名： 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
说明书
引用文献 (1)
评论 (3)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Ponceau S Staining Solution

Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting.

技术数据 for Ponceau S Staining Solution

分子量 760.57
公式 C22H12N4Na4O13S4
储存 Store at RT
CAS Number 6226-79-5
PubChem ID 101933731
InChI Key KQHKSGRIBYJYFX-UHFFFAOYSA-J
Smiles OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Ponceau S Staining Solution

溶解性 Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution)

产品说明书 for Ponceau S Staining Solution

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参考文献 for Ponceau S Staining Solution

参考文献是支持产品生物活性的出版物。

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关键词: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience

1 篇 Ponceau S Staining Solution 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Ponceau S Staining Solution 的部分引用包括：

Ronald L et al (2023) Early Mitochondrial Defects in the 5xFAD Mouse Model of Alzheimer's Disease. J Alzheimers Dis 91 1323-1338 PMID: 36617782

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Ponceau S Staining Solution 的评论

平均评分: 4.3 (基于 3 条评论。)

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Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation..
By Anonymous on 12/23/2019
分析类型： In Vitro
种属： Human
细胞系/组织： HEK293

Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).

The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.

review image
Best staining for tracking protein transfer in western blot.
By Luiz Alexandre Magno on 07/03/2018
分析类型： In Vitro
种属： Mouse

We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.

1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections

PMID: 26585834
review image
Western blot confirmation.
By Anonymous on 01/05/2018
分析类型： In Vitro
种属： Human

Western blot confirmation

该领域的文献

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