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Puromycin dihydrochloride

Cat. No. 4089 16 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Protein synthesis inhibitor
化学名： 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
纯度： ≥98% (HPLC)
说明书
引用文献 (16)
评论 (1)
文献 (2)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Puromycin dihydrochloride

Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.

技术数据 for Puromycin dihydrochloride

分子量 544.43
公式 C22H29N7O5.2HCl
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 58-58-2
PubChem ID 443311
InChI Key MKSVFGKWZLUTTO-FZFAUISWSA-N
Smiles O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Puromycin dihydrochloride

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
water 54.44 100
DMSO 54.44 100

制备储备液 for Puromycin dihydrochloride

以下数据基于产品分子量 544.43。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 1.84 mL 9.18 mL 18.37 mL
5 mM 0.37 mL 1.84 mL 3.67 mL
10 mM 0.18 mL 0.92 mL 1.84 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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产品说明书 for Puromycin dihydrochloride

分析证书/产品说明书
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化学品安全技术说明书

参考文献 for Puromycin dihydrochloride

参考文献是支持产品生物活性的出版物。

Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141

Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866 PMID: 4590173

If you know of a relevant reference for Puromycin dihydrochloride, please let us know.

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关键词: Puromycin dihydrochloride, Puromycin dihydrochloride supplier, Puromycin, antibiotics, protein, synthesis, inhibitors, inhibits, translation, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4089, Tocris Bioscience

16 篇 Puromycin dihydrochloride 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Puromycin dihydrochloride 的部分引用包括：

Dobrikov et al (2014) Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol 34 439 PMID: 24248602

Dasgupta et al (2014) Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells. PLoS One 9 e103604 PMID: 25079948

Zhu et al (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. J Neurosci 10 e0143255 PMID: 26571119

Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33453847

Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33082139

Mark L et al (2020) Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation. Cell Rep 31 107785 PMID: 32579917

Philipp et al (2020) An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells. Cancers (Basel) 12 PMID: 32580291

Leung et al (2016) WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143 398 PMID: 26839343

Luke A et al (2016) Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Elife 5 PMID: 27661255

Dasgupta et al (2015) Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease. PLoS One 10 e0145333 PMID: 26691307

Tao et al (2021) CT45A1 promotes the metastasis of osteosarcoma cells in vitro and in vivo through β-catenin. Cell Death Dis 12 650 PMID: 34172717

Balaji M et al (2021) Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J Biol Chem 296 100386 PMID: 33556374

Nabil G et al (2021) PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs. Stem Cell Reports 16 2958-2972 PMID: 34739847

Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407

Yu et al (2019) Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. PLoS One 14 e0210608 PMID: 30625226

Ma et al (2017) AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma. Am J Transl Res. 9 507 PMID: 28337279

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Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells..
By Niroj Shrestha on 01/20/2019
分析类型： In Vitro
种属： Human
细胞系/组织： HEK293

Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.

Puromycin should be stored in aliquots at -20 degree C.

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