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说明： Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
别名： TADA
纯度： ≥98% (HPLC)
生物活性 for RADA
RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.
This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Scientific Data
Fluorescence Imaging
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Application of RADA. Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale: 5 μm.
For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841
Optical Data for RADA
| Emission Color | Orange |
|---|---|
| λabs | 554 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Application | dSTORM, reSTORM |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for RADA
| 分子量 | 516.55 |
| 公式 | C28H28N4O6 |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| InChI Key | SMFYRQAHWDWBTD-AVHJOICASA-N |
| Smiles | N[C@@H](C(O)=O)CNC(C1=CC(C(C2=C(C=C(N(C)C)C=C2)O3)=C4C3=C/C(C=C4)=[N+](C)\C)=C(C=C1)C([O-])=O)=O.N[C@@H](C(O)=O)CNC(C5=CC(C([O-])=O)=C(C(C6=C(C=C(N(C)C)C=C6)O7)=C8C7=C/C(C=C8)=[N+](C)\C)C=C5)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for RADA
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 51.66 | 100 |
制备储备液 for RADA
以下数据基于产品分子量 516.55。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.68 mL | 19.36 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.87 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
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产品说明书 for RADA
参考文献 for RADA
参考文献是支持产品生物活性的出版物。
Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665
García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 7 pii: e37243. PMID: 30198841
Fenton et al (2017) Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol. 2 17011 PMID: 28112724
If you know of a relevant reference for RADA, please let us know.
按标靶查看相关产品
关键词: RADA, RADA supplier, Fluorescent, D-amino, acid, FDAA, TADA, TAMRA, labeling, peptidoglycans, bacteria, dSTORM, reSTORM, SRM, super, resolution, microscopy, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6649, Tocris Bioscience
2 篇 RADA 的引用文献
引用文献是使用了 Tocris 产品的出版物。 RADA 的部分引用包括：
Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841
Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals