Rf470DL

Pricing Availability   Qty
说明: Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria
化学名: N6-[3-[6-[5-[2-Carboxy-2-cyanoethenyl]-2-thienyl]-3,4-dihydro-1(2H)-quinolinyl]-1-oxopropyl]-D-lysine hydrochloride
纯度: ≥95% (HPLC)
说明书
引用文献
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文献 (1)

生物活性 for Rf470DL

Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).

许可信息

Sold under license from Indiana University. U.S. patent application number 16/967,874.

Optical Data for Rf470DL

Emission Color Red
λabs 470 nm
λem 640 nm
Extinction Coefficient (ε) 33106 M-1cm-1
Quantum Yield (φ) 0.042
Application Fluorogenic Probe for Imaging Bacterial Cell Wall Biosynthesis (PG Biosynthesis)

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技术数据 for Rf470DL

分子量 547.07
公式 C26H30N4O5S.HCl
储存 Store at -20°C
纯度 ≥95% (HPLC)
InChI Key ITTDOARQMPIHGH-IVUCCOTMSA-N
Smiles N[C@@H](C(O)=O)CCCCNC(CCN1CCCC2=CC(C3=CC=C(/C=C(C(O)=O)\C#N)S3)=CC=C12)=O.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Rf470DL

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 54.71 100

制备储备液 for Rf470DL

以下数据基于产品分子量 547.07。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 1.83 mL 9.14 mL 18.28 mL
5 mM 0.37 mL 1.83 mL 3.66 mL
10 mM 0.18 mL 0.91 mL 1.83 mL
50 mM 0.04 mL 0.18 mL 0.37 mL

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参考文献 for Rf470DL

参考文献是支持产品生物活性的出版物。

Hsu et al (2019) Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 11 335 PMID: 30804500


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