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Submit ReviewRf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).
Sold under license from Indiana University. U.S. patent application number 16/967,874.
Emission Color | Red |
---|---|
λabs | 470 nm |
λem | 640 nm |
Extinction Coefficient (ε) | 33106 M-1cm-1 |
Quantum Yield (φ) | 0.042 |
Application | Fluorogenic Probe for Imaging Bacterial Cell Wall Biosynthesis (PG Biosynthesis) |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 547.07 |
公式 | C26H30N4O5S.HCl |
储存 | Store at -20°C |
纯度 | ≥95% (HPLC) |
InChI Key | ITTDOARQMPIHGH-IVUCCOTMSA-N |
Smiles | N[C@@H](C(O)=O)CCCCNC(CCN1CCCC2=CC(C3=CC=C(/C=C(C(O)=O)\C#N)S3)=CC=C12)=O.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 54.71 | 100 |
以下数据基于产品分子量 547.07。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.83 mL | 9.14 mL | 18.28 mL |
5 mM | 0.37 mL | 1.83 mL | 3.66 mL |
10 mM | 0.18 mL | 0.91 mL | 1.83 mL |
50 mM | 0.04 mL | 0.18 mL | 0.37 mL |
参考文献是支持产品生物活性的出版物。
Hsu et al (2019) Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 11 335 PMID: 30804500
If you know of a relevant reference for Rf470DL, please let us know.
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