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Submit ReviewXIAP Tracer mF-Smac 最新
说明： Fluorescein labeled XIAP ligand for TR-FRET assay development
纯度： ≥95% (HPLC)
生物活性 for XIAP Tracer mF-Smac
XIAP Tracer mF-Smac is a fluorescent labeled peptide used as an acceptor dye with a terbium labeled BIR domain antibody in TR-FRET assays (Kd values are 4.7 nM at BIR3 (XIAP); 17.9 nM in XIAP polarization assay).
Optical Data for XIAP Tracer mF-Smac
| Emission Color | Near-IR |
|---|---|
| λabs | 485 nm |
| λem | 520 nm |
| Closest Laser line | 488 nm |
| Application | Fluorescence polarization (FP), Time-resolved fluorescence energy transfer (TR-FRET) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for XIAP Tracer mF-Smac
| 分子量 | 989.1 |
| 公式 | C51H60N10O11 |
| 序列 |
XRPFK (Modifications: X-1 = 2-Aminobutanoic acid, Lys-5 = Lys(5-FAM), Lys-5 = C-terminal amide) |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 783340-88-5 |
| InChI Key | ONHYLEJPWIONKB-HECCNADXSA-N |
| Smiles | CC[C@H](N)C(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(N)=O)CCCCNC(C2=CC=C3C(C(OC34C5=CC=C(O)C=C5OC6=CC(O)=CC=C64)=O)=C2)=O)=O)CC7=CC=CC=C7)=O)=O)CCCNC(N)=N)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for XIAP Tracer mF-Smac
| 溶解性 | Soluble to 2 mg/ml in 25% ethanol / water |
产品说明书 for XIAP Tracer mF-Smac
参考文献 for XIAP Tracer mF-Smac
参考文献是支持产品生物活性的出版物。
Chaudhry et al (2016) Building homogeneous time-resolved fluorescence resonance energy transfer assays for characterization of bivalent inhibitors of an inhibitor of apoptosis protein target. Anal.Biochem. 497 8 PMID: 26743718
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Pathways for XIAP Tracer mF-Smac
