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Submit ReviewY-27632 in solution is a convenient and ready-to-use 10 mM sterile-filtered solution of Y-27632 (Cat. No. 1254), pre-dissolved in water. Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as a component of growth media for urothelial organoids and in a protocol to generate brain organoids from human iPSCs.
Supplied in vials containing a minimum of 1 mL of Y-27632 solution.
分子量 | 320.26 |
公式 | C14H21N3O.2HCl |
储存 | Store at -20°C |
纯度 | ≥97% (HPLC) |
CAS Number | 129830-38-2 |
PubChem ID | 9901617 |
InChI Key | IDDDVXIUIXWAGJ-LJDSMOQUSA-N |
Smiles | N[C@H](C)[C@@]1([H])CC[C@H](C(NC2=CC=NC=C2)=O)CC1.Cl.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性 | Soluble in water (supplied pre-dissolved at a concentration of 10mM) |
参考文献是支持产品生物活性的出版物。
Schafer et al (2023) An in vivo neuroimmune organoid model to study human microglia phenotypes. Cell 186 2111 PMID: 37172564
Wilson et al (2016) Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition. Tissue.Eng.Part C.Methods 22 1085 PMID: 27846787
Ichikawa et al (2011) Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling. Cryo Letters 32 516 PMID: 22227712
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Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意,Tocris 仅会向正规科研企业/机构地址发送文献。
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.