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Submit ReviewPhorbol 12,13-dibutyrate
Description: Protein kinase C activator
Alternative Names: PDBu
Chemical Name: (1aR,1bS,4aR,7aS,7bS,8R,9R,9aS)-1a,1b,4,4a,5,7a,7b,8,9,9a-Decahydro-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9,9a-diyl butanoic acid ester
Purity: ≥97% (HPLC)
Biological Activity for Phorbol 12,13-dibutyrate
Phorbol 12,13-dibutyrate is a protein kinase C activator. Induces contraction of vascular smooth muscle and inhibits MLC phosphatase (MLCP) in vascular smooth muscle. Activity does not alter intracellular Ca2+ concentration. Also inhibits the activity of Na+,K+ ATPase in OK cells.
For more information about how Phorbol 12,13-dibutyrate may be used, see our protocol: Generation of β cells from hPSCs
Technical Data for Phorbol 12,13-dibutyrate
| M. Wt | 504.61 |
| Formula | C28H40O8 |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 37558-16-0 |
| PubChem ID | 37783 |
| InChI Key | BQJRUJTZSGYBEZ-YVQNUNKESA-N |
| Smiles | OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(CCC)=O)[C@H](OC(CCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Phorbol 12,13-dibutyrate
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 25.23 | 50 |
Preparing Stock Solutions for Phorbol 12,13-dibutyrate
The following data is based on the product molecular weight 504.61. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 3.96 mL | 19.82 mL | 39.63 mL |
| 2.5 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 5 mM | 0.4 mL | 1.98 mL | 3.96 mL |
| 25 mM | 0.08 mL | 0.4 mL | 0.79 mL |
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Product Datasheets for Phorbol 12,13-dibutyrate
References for Phorbol 12,13-dibutyrate
References are publications that support the biological activity of the product.
Middleton et al (1993) Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells. J.Biol.Chem. 268 15958 PMID: 8393456
Hori et al (1999) Presynaptic mechanism for phorphol ester-induced synaptic potentiation. J.Neurosci. 19 7262 PMID: 10460232
Sakai et al (2010) Augmented PDBu-mediated contaction of bronchial smooth muscle of mice with antigen-induced airway hyperresponsiveness. J.Smooth Muscle Res. 46 259 PMID: 21187674
If you know of a relevant reference for Phorbol 12,13-dibutyrate, please let us know.
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Keywords: Phorbol 12,13-dibutyrate, Phorbol 12,13-dibutyrate supplier, PDBu, protein, kinases, c, pkc, activators, phorbol, esters, Protein, Kinase, C, Enzyme, Substrates, /, Activators, 4153, Tocris Bioscience
11 Citations for Phorbol 12,13-dibutyrate
Citations are publications that use Tocris products. Selected citations for Phorbol 12,13-dibutyrate include:
Nieuwenhuijze et al (2018) Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations. Front Immunol 9 2234 PMID: 30323813
Nostro et al (2015) Efficient generation of NKX6-1+ pancreatic progenitors from multiple human pluripotent stem cell lines. J Cell Biol 4 591 PMID: 25843049
Sarah C et al (2021) A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice. Med (N Y) 2 243-262.e8 PMID: 33521747
Timothy S et al (2021) Differential Regulation of ATP- and UTP-Evoked Prostaglandin E2 and IL-6 Production from Human Airway Epithelial Cells. J Immunol 207 1275-1287 PMID: 34389624
Yi et al (2012) Constriction of retinal arterioles to endothelin-1: requisite role of rho kinase independent of protein kinase C and L-type calcium channels. Invest Ophthalmol Vis Sci 53 2904-12 PMID: 22427601
Sánchez et al (2014) Endothelin-1 contributes to endothelial dysfunction and enhanced vasoconstriction through augmented superoxide production in penile arteries from Ins-resistant obese rats: role of ET(A) and ET(B) receptors. Br J Pharmacol 171 5682 PMID: 25091502
Gareth E et al (2022) Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv 8 eabm3471 PMID: 35427166
Oliver T et al (2022) Do more with Less: Improving High Parameter Cytometry Through Overnight Staining. Curr Protoc 2 e589 PMID: 36373983
Nidhi et al (2020) Neonatal-derived IL-17 producing dermal γδ T cells are required to prevent spontaneous atopic dermatitis. Elife 9 PMID: 32065580
Xavier et al (2020) BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells. Cell Death Dis 11 378 PMID: 32424151
Zoltan et al (2020) A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Rep 32 107968 PMID: 32726631
Do you know of a great paper that uses Phorbol 12,13-dibutyrate from Tocris? Please let us know.
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PDB reduces saxagliptin-induced APD prolongation in cardiomyocytes.
By
Chintan Koyani on 10/22/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: ventricular cardiomyocytes
Guinea pig ventricular cardiomyocytes were patched and superfused with Saxagliptin (2 µM, 5 min) followed by Phorbol 12,13-dibutyrate (PDB, 10 nM, 5 min) and action potentials were stimulated at a 1 Hz frequency. Action potential duration (APD) were analysed at 20%, 50% and 90% repolarization (shown in Image). PDB reduced saxagliptin-induced APD prolongation.
