Home / Cell Biology / Cell Metabolism / DNA, RNA and Protein Synthesis / 5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2'-deoxyuridine

Cat. No. 7207 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Thymidine analog for labeling DNA; can be linked to a fluorophore by click chemistry
Chemical Name: 5-ethynyl-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for 5-Ethynyl-2'-deoxyuridine

5-Ethynyl-2'-deoxyuridine is a thymidine analog for labeling DNA. Can be linked to an azide fluorophore, such as 3-Azido-7-hydroxycoumarin (Cat. No. 7664) through click chemistry and used to detect DNA synthesis in proliferating cells in vivo and in vitro. Detects very low levels of cell proliferation in tissues with low cellular turnover. 5-Ethynyl-2'-deoxyuridine suppresses cell proliferation in osteosarcoma and glioblastoma cell lines, induces a 'futile' DNA repair cycle and induces DNA strand breaks and apoptosis at a higher rate than Temozolomide (Cat. No. 2706).

Technical Data for 5-Ethynyl-2'-deoxyuridine

M. Wt 252.23
Formula C11H12N2O5
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 61135-33-9
PubChem ID 472172
InChI Key CDEURGJCGCHYFH-DJLDLDEBSA-N
Smiles O=C1N([C@@]2(O[C@H](CO)[C@@H](O)C2)[H])C=C(C#C)C(=O)N1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for 5-Ethynyl-2'-deoxyuridine

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 25.22 100

Preparing Stock Solutions for 5-Ethynyl-2'-deoxyuridine

The following data is based on the product molecular weight 252.23. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.96 mL 19.82 mL 39.65 mL
5 mM 0.79 mL 3.96 mL 7.93 mL
10 mM 0.4 mL 1.98 mL 3.96 mL
50 mM 0.08 mL 0.4 mL 0.79 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for 5-Ethynyl-2'-deoxyuridine

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for 5-Ethynyl-2'-deoxyuridine

References are publications that support the biological activity of the product.

Salic & Mitchison (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc.Natl.Acad.Sci.U.S.A. 105 2415 PMID: 18272492

Wang et al (2022) Nucleotide excision repair removes thymidine analog 5-ethynyl-2'-deoxyuridine from the mammalian genome. Proc.Natl.Acad.Sci.U.S.A. 119 e2210176119 PMID: 35994676

If you know of a relevant reference for 5-Ethynyl-2'-deoxyuridine, please let us know.

View Related Products by Target

Keywords: 5-Ethynyl-2'-deoxyuridine, 5-Ethynyl-2'-deoxyuridine supplier, 5Ethynyl2deoxyuridine, thymidine, analog, analogue, DNA, labeling, labelling, click, chemistry, cell, proliferation, osteosarcoma, glioblastoma, apoptosis, DNA,, RNA, and, Protein, Synthesis, 7207, Tocris Bioscience

Citations for 5-Ethynyl-2'-deoxyuridine

Citations are publications that use Tocris products.

Currently there are no citations for 5-Ethynyl-2'-deoxyuridine. Do you know of a great paper that uses 5-Ethynyl-2'-deoxyuridine from Tocris? Please let us know.

Reviews for 5-Ethynyl-2'-deoxyuridine

There are currently no reviews for this product. Be the first to review 5-Ethynyl-2'-deoxyuridine and earn rewards!

Have you used 5-Ethynyl-2'-deoxyuridine?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:

  • Cell Cycle and Mitosis
  • DNA Damage Repair
  • Targeted Protein Degradation
  • Ubiquitin Proteasome Pathway
  • Chemotherapy Targets
Huntington's Disease Research Product Guide

Huntington's Disease Research Product Guide

This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:

  • Somatic Instability
  • Proteolysis and Inclusion Bodies
  • Transcriptional Dysregulation
  • Mitochondrial Dysfunction
  • Nuclear-Cytoplasmic Transport Interference
  • Excitotoxicity
  • Stem Cells