Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewAbK
Description: Photo-crosslinking probe; unnatural amino acid
Chemical Name: N6-[[2-(3-Methyl-3H-diazirin-3-yl)ethoxy]carbonyl]-L-lysine
Purity: ≥98% (HPLC)
Biological Activity for AbK
AbK is an unnatural amino acid. Acts as a UV light-activated (369 nm) photocrosslinking probe when incorporated into proteins by pyrrolysyl tRNA synthetase/tRNACUA pairs.
Technical Data for AbK
| M. Wt | 272.3 |
| Formula | C11H20N4O4.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1253643-88-7 |
| PubChem ID | 66825403 |
| InChI Key | LUCMNTLJAFTFDU-QMMMGPOBSA-N |
| Smiles | N[C@H](C(O)=O)CCCCNC(OCCC1(C)N=N1)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for AbK
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 2.72 | 10 with gentle warming | |
| 1eq. HCl | 27.23 | 100 |
Preparing Stock Solutions for AbK
The following data is based on the product molecular weight 272.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.67 mL | 18.36 mL | 36.72 mL |
| 5 mM | 0.73 mL | 3.67 mL | 7.34 mL |
| 10 mM | 0.37 mL | 1.84 mL | 3.67 mL |
| 50 mM | 0.07 mL | 0.37 mL | 0.73 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for AbK
References for AbK
References are publications that support the biological activity of the product.
Ai et al (2011) Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem 12 1854 PMID: 21678540
Chatterjee et al (2013) Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc.Natl.Acad.Sci.U.S.A. 110 11803 PMID: 23818609
Lowder et al (2011) Visualizing protein partnerships in living cells and organisms. Curr.Opin.Cell Biol. 15 781 PMID: 22104179
Kleiner et al (2018) A chemical proteomics approach to reveal direct protein-protein interactions in living cells. Cell Chem.Biol. 25 110 PMID: 29104064
If you know of a relevant reference for AbK, please let us know.
View Related Products by Target
Keywords: AbK, AbK supplier, photo-crosslinking, amino, acids, UV, light, tRNA, synthetase, pairs, reagents, photocrosslinking, unnatural, acid, UAA, Biochemicals, and, Molecular, Biology, Unnatural, Amino, Acids, Photoactivatable, cross-linking, probes, 5113, Tocris Bioscience
1 Citation for AbK
Citations are publications that use Tocris products. Selected citations for AbK include:
Syed et al (2019) Expanding the Zebrafish Genetic Code through Site-Specific Introduction of Azido-lysine, Bicyclononyne-lysine, and Diazirine-lysine. Int J Mol Sci 20 PMID: 31130675
Do you know of a great paper that uses AbK from Tocris? Please let us know.
Reviews for AbK
There are currently no reviews for this product. Be the first to review AbK and earn rewards!
Have you used AbK?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image