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Description: Fluorescent ALDH substrate and detection reagent. Used for: identification of cells with high ALDH activity. Application: flow cytometry, fluorescent-activated cell sorting (FACS)
Chemical Name: (T-4)-[N-(2,2-Diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
Purity: ≥95% (HPLC)
Biological Activity for ALDH Detection Reagent - BAAA-DA
Key information: ALDH Detection Reagent - BAAA-DA is a fluorescent substrate of aldehyde dehydrogenase (ALDH).
Used for: can be used in flow cytometry to sort cancer stem cells with high ALDH levels, and to isolate human hematopoietic progenitor cells. Can be used with efflux inhibitors such as Verapamil (Cat. No. 0654), Probenecid (Cat. No. 4107) or 2-deoxy-D-glucose (Cat. No. 4515) to block multi-drug resistance efflux.
Application: flow cytometry, fluorescent-activated cell sorting (FACS).
Properties and Photophysical Data: BAAA-DA is converted under acidic conditions to BAAA. BAAA, cell-permeant, is converted by intracellular ALDH into carboxylate ion amino acetate (BAA) and used to identify cells with high ALDH activity. Excitation and emission maxima (λ) are 505 nm and 512 nm, respectively; extinction coefficient = 74,595 M-1cm-1.
Optical Data for ALDH Detection Reagent - BAAA-DA
| λabs | 505 nm |
|---|---|
| λem | 512 nm |
| Extinction Coefficient (ε) | 74595 M-1cm-1 |
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Technical Data for ALDH Detection Reagent - BAAA-DA
| M. Wt | 407.27 |
| Formula | C20H28BF2N3O3 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 247069-93-8 |
| PubChem ID | 91759536 |
| InChI Key | LHPURSCMUYRRRM-UHFFFAOYSA-N |
| Smiles | CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCC(OCC)OCC)=O)C=CC3=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ALDH Detection Reagent - BAAA-DA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.07 | 10 |
Preparing Stock Solutions for ALDH Detection Reagent - BAAA-DA
The following data is based on the product molecular weight 407.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 24.55 mL | 122.77 mL | 245.54 mL |
| 0.5 mM | 4.91 mL | 24.55 mL | 49.11 mL |
| 1 mM | 2.46 mL | 12.28 mL | 24.55 mL |
| 5 mM | 0.49 mL | 2.46 mL | 4.91 mL |
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Product Datasheets for ALDH Detection Reagent - BAAA-DA
References for ALDH Detection Reagent - BAAA-DA
References are publications that support the biological activity of the product.
Storms et al (1999) Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc.Natl.Acad.Sci.U.S.A. 96 9118 PMID: 10430905
If you know of a relevant reference for ALDH Detection Reagent - BAAA-DA, please let us know.
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