Home / Cell Biology / Cell Metabolism / DNA, RNA and Protein Synthesis / DNA, RNA and Protein Synthesis Antagonists / Auxinole

Auxinole

Cat. No. 7894 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Auxin antagonist of TIR1/AFB receptors; also OsTIR1 inhibitor in auxin-inducible degron (AID) system
Chemical Name: α-[2-(2,4-Dimethylphenyl)-2-oxoethyl]-1H-indole-3-acetic acid
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Auxinole

Auxinole is an auxin antagonist of TIR1/AFB receptors. Binds to TIR1 to block the formation of the TIR1-IAA-Aux/IAA complex and inhibit auxin-responsive gene expression. Auxinole competitively inhibits various auxin responses in planta. Auxinole also inhibits OsTIR1 that suppresses leaky degradation of degron-fused proteins. Can be used as a control compound with 5-Ph-IAA (Cat. No. 7392) in the AID2 degron system to switch off degradation.

Technical Data for Auxinole

M. Wt 321.37
Formula C20H19NO3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 86445-22-9
PubChem ID 13077496
InChI Key HGUYAIJBXSQXGV-UHFFFAOYSA-N
Smiles O=C(O)C(C1=CNC=2C=CC=CC21)CC(=O)C3=CC=C(C=C3C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Auxinole

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.14 100
ethanol 6.43 20 with gentle warming

Preparing Stock Solutions for Auxinole

The following data is based on the product molecular weight 321.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.11 mL 15.56 mL 31.12 mL
5 mM 0.62 mL 3.11 mL 6.22 mL
10 mM 0.31 mL 1.56 mL 3.11 mL
50 mM 0.06 mL 0.31 mL 0.62 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for Auxinole

References are publications that support the biological activity of the product.

Hayashi et al (2012) Rational design of an auxin antagonist of the SCF(TIR1) auxin receptor complex. ACS Chem.Biol. 7 590 PMID: 22234040

Yesbolatova et al (2019) Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Methods 164 73 PMID: 31026591

If you know of a relevant reference for Auxinole, please let us know.

View Related Products by Target

View Related Products by Product Action

View all DNA, RNA and Protein Synthesis Antagonists

Keywords: Auxinole, Auxinole supplier, Auxin, AID, targeted, protein, degradation, degron, TPD, chemical, TIR1, phytohormone, degrade, degraders, DNA,, RNA, and, Protein, Synthesis, Molecular, Glues, 7894, Tocris Bioscience

Citations for Auxinole

Citations are publications that use Tocris products.

Currently there are no citations for Auxinole. Do you know of a great paper that uses Auxinole from Tocris? Please let us know.

Reviews for Auxinole

There are currently no reviews for this product. Be the first to review Auxinole and earn rewards!

Have you used Auxinole?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Cell Cycle and DNA Damage Research Product Guide

Cell Cycle and DNA Damage Research Product Guide

This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:

  • Cell Cycle and Mitosis
  • DNA Damage Repair
  • Targeted Protein Degradation
  • Ubiquitin Proteasome Pathway
  • Chemotherapy Targets
Huntington's Disease Research Product Guide

Huntington's Disease Research Product Guide

This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:

  • Somatic Instability
  • Proteolysis and Inclusion Bodies
  • Transcriptional Dysregulation
  • Mitochondrial Dysfunction
  • Nuclear-Cytoplasmic Transport Interference
  • Excitotoxicity
  • Stem Cells