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Submit ReviewIRDye® 680RD, NHS ester New
Description: Near-infared dye (reactive group NHS ester)
Chemical Name: Sodium (Z)-2-((2Z,4E)-5-(5-Chloro-3,3-dimethyl-7-(3-sulfonatopropyl)-3H-pyrrolo[2,3-b]pyridin-7-ium-2-yl)-3-(3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)penta-2,4-dien-1-ylidene)-3,3-dimethyl-1-(3-sulfonatopropyl)indoline-5-sulfonate
Purity: ≥70% (HPLC)
Biological Activity for IRDye® 680RD, NHS ester
Key information: IRDye® 680RD, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
NOTE: The choice of solvent for the dye is dependent on the stability of the protein in DMSO. Since a small amount of dye is needed to label large (i.e. >45 kDA) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDA), the level of DMSO in the reaction is more likely to affect the protein stability. Dye solubilized in water should be discarded after one use. Dye prepared in DMSO is stable for up to two weeks when stored at -20°C and protected from light and moisture.
It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)
IRDye® is a registered trademark of Li-Cor and is used under license.
Optical Data for IRDye® 680RD, NHS ester
| λabs | 680 nm |
|---|---|
| λem | 694 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for IRDye® 680RD, NHS ester
| M. Wt | 1003.46 |
| Formula | C43H45ClN4Na2O13S3 |
| Storage | Store at -20°C |
| Purity | ≥70% (HPLC) |
| CAS Number | 1373919-87-9 |
| InChI Key | JNFTWCVWRDPJNM-UHFFFAOYSA-L |
| Smiles | O=S(C1=CC(C/2(C)C)=C(C=C1)N(CCCS(=O)(O[Na])=O)C2=C/C=C(C3=CC(CCC(ON4C(CCC4=O)=O)=O)=CC=C3)/C=C/C5=NC6=[N+](CCCS([O-])(=O)=O)C=C(Cl)C=C6C5(C)C)(O[Na])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for IRDye® 680RD, NHS ester
References for IRDye® 680RD, NHS ester
References are publications that support the biological activity of the product.
Al-Hawat et al (2024) Development of a polymersome blood ammonia assay coupled with a portable near-infrared fluorometer. ACS Bio.Med.Chem.Au. 4 226 PMID: 39431266
If you know of a relevant reference for IRDye® 680RD, NHS ester, please let us know.
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Keywords: IRDye® 680RD, NHS ester, IRDye® 680RD, NHS ester supplier, NIR, near, infrared, short, wave, SWIR, fluorescent, dyes, fluorescence, imaging, labeling, labelling, Near, Infrared, Fluorescent, (NIR), Dyes, 8878, Tocris Bioscience
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