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Submit ReviewIRDye® 680RD, NHS ester New
Description: Near-infared dye (reactive group NHS ester)
Chemical Name: Sodium (Z)-2-((2Z,4E)-5-(5-Chloro-3,3-dimethyl-7-(3-sulfonatopropyl)-3H-pyrrolo[2,3-b]pyridin-7-ium-2-yl)-3-(3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)penta-2,4-dien-1-ylidene)-3,3-dimethyl-1-(3-sulfonatopropyl)indoline-5-sulfonate
Purity: ≥70% (HPLC)
Biological Activity for IRDye® 680RD, NHS ester
Key information: IRDye® 680RD, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)
Optical Data for IRDye® 680RD, NHS ester
| λabs | 680 nm |
|---|---|
| λem | 694 nm |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for IRDye® 680RD, NHS ester
| M. Wt | 1003.46 |
| Formula | C43H45ClN4Na2O13S3 |
| Storage | Store at -20°C |
| Purity | ≥70% (HPLC) |
| CAS Number | 1373919-87-9 |
| InChI Key | JNFTWCVWRDPJNM-UHFFFAOYSA-L |
| Smiles | O=S(C1=CC(C/2(C)C)=C(C=C1)N(CCCS(=O)(O[Na])=O)C2=C/C=C(C3=CC(CCC(ON4C(CCC4=O)=O)=O)=CC=C3)/C=C/C5=NC6=[N+](CCCS([O-])(=O)=O)C=C(Cl)C=C6C5(C)C)(O[Na])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for IRDye® 680RD, NHS ester
References for IRDye® 680RD, NHS ester
References are publications that support the biological activity of the product.
Al-Hawat et al (2024) Development of a polymersome blood ammonia assay coupled with a portable near-infrared fluorometer. ACS Bio.Med.Chem.Au. 4 226 PMID: 39431266
If you know of a relevant reference for IRDye® 680RD, NHS ester, please let us know.
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Keywords: IRDye® 680RD, NHS ester, IRDye® 680RD, NHS ester supplier, NIR, near, infrared, short, wave, SWIR, fluorescent, dyes, fluorescence, imaging, labeling, labelling, Near, Infrared, Fluorescent, (NIR), Dyes, 8878, Tocris Bioscience
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