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Submit ReviewJanelia Fluor® 646, Tetrazine
Description: Red dye (Reactive group: tetrazine). Application: flow cytometry, confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
Alternative Names: JF646
Chemical Name: 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥90% (HPLC)
Biological Activity for Janelia Fluor® 646, Tetrazine
Key Information: Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two-color imaging with Janelia Fluor;® 549 Tetrazine (Cat. No. 6502). Cell-permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, Tetrazine
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 647 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.19 |
| Application | Confocal Microscopy, SRM, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data for Janelia Fluor® 646, Tetrazine
| M. Wt | 665.83 |
| Formula | C38H35N7O3Si |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 2042192-00-5 |
| InChI Key | CPWVRCGDSYQFSV-UHFFFAOYSA-N |
| Smiles | C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=CC(C(NCC6=CC=C(C=C6)C7=NN=CN=N7)=O)=CC=C5C([O-])=O)=C1C=CC2=[N+]8CCC/8 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 646, Tetrazine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.32 | 20 |
Preparing Stock Solutions for Janelia Fluor® 646, Tetrazine
The following data is based on the product molecular weight 665.83. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.51 mL | 37.55 mL | 75.09 mL |
| 1 mM | 1.5 mL | 7.51 mL | 15.02 mL |
| 2 mM | 0.75 mL | 3.75 mL | 7.51 mL |
| 10 mM | 0.15 mL | 0.75 mL | 1.5 mL |
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Product Datasheets for Janelia Fluor® 646, Tetrazine
References for Janelia Fluor® 646, Tetrazine
References are publications that support the biological activity of the product.
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 646, Tetrazine, please let us know.
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1 Citation for Janelia Fluor® 646, Tetrazine
Citations are publications that use Tocris products. Selected citations for Janelia Fluor® 646, Tetrazine include:
Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914
Do you know of a great paper that uses Janelia Fluor® 646, Tetrazine from Tocris? Please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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