LFM-A13

Cat. No. 1300 4 Citations Submit a Review Datasheet / COA / SDS

Pricing	Availability		Qty

Description: Potent, selective BTK inhibitor

Chemical Name: 2-Cyano-N-(2,5-dibromophenyl)-3-hydroxy-2-butenamide

Purity: ≥98% (HPLC)

Datasheet

Citations (4)

Reviews

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for LFM-A13

LFM-A13 is a potent and selective inhibitor of Bruton's tyrosine kinase (BTK). Inhibits recombinant BTK with an IC₅₀ value of 2.5 μM and has no activity on other protein kinases ( including JAK1, JAK3, HCK, EGFR kinase and insulin receptor kinase) at concentrations of up to 278 μM.

Compound Libraries for LFM-A13

LFM-A13 is also offered as part of the Tocriscreen Kinase Inhibitor Library. Find out more about compound libraries available from Tocris.

Technical Data for LFM-A13

M. Wt	360
Formula	C₁₁H₈Br₂N₂O₂
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	62004-35-7
PubChem ID	54676905
InChI Key	UVSVTDVJQAJIFG-VURMDHGXSA-N
Smiles	C\C(O)=C(/C#N)C(=O)NC1=C(Br)C=CC(Br)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for LFM-A13

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	36	100

Preparing Stock Solutions for LFM-A13

The following data is based on the product molecular weight 360. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.78 mL	13.89 mL	27.78 mL
5 mM	0.56 mL	2.78 mL	5.56 mL
10 mM	0.28 mL	1.39 mL	2.78 mL
50 mM	0.06 mL	0.28 mL	0.56 mL

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Product Datasheets for LFM-A13

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for LFM-A13

References are publications that support the biological activity of the product.

Crosby and Pool (2002) Interaction of Bruton's tyrosine kinase and protein kinase Cθ in platelets. J.Biol.Chem. 277 9958 PMID: 11788586

Mahajan et al (1999) Rational design and synthesis of a novel anti-leukemic agent targeting Bruton's tyrosine kinase (BTK), LFM-A13 [α-cyano-β-hydroxy-β-methyl-N-(2,5-dibromophenyl)propenamide]. J.Biol.Chem. 274 9587 PMID: 10092645

Vassilev et al (1999) Bruton's tyrosine kinase as an inhibitor of the Fas/CD95 death-inducing signaling complex. J.Biol.Chem. 274 1646 PMID: 9880544

If you know of a relevant reference for LFM-A13, please let us know.

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