Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewLin28 1632
Description: RNA binding protein Lin28 inhibitor; promotes mESC differentiation; also bromodomain inhibitor
Chemical Name: N-Methyl-N-[3-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)phenyl]acetamide
Purity: ≥98% (HPLC)
Biological Activity for Lin28 1632
Lin28 1632 is an RNA binding protein Lin28 inhibitor (IC50 = 8 μM). Lin28 1632 blocks binding of Lin28 to let-7 pre-micro RNA. Lin28 1632 inhibits stemness and induces differentiation of murine ESCs. Lin28 1632 increases let-7 miRNA and suppresses PD-L1 expression in multiple human cancer cell lines. Lin28 1632 inhibits growth of tumor cell lines and tumor-sphere formation in vitro. Lin28 1632 also inhibits growth of tumor xenografts in mice by enhancing T cell-mediated antitumor immunity. Lin28 1632 is also bromodomain inhibitor. Lin28 1632 inhibits tumor growth and metastasis in vivo in immunodeficient orthotopic mouse models of human breast cancer cell MDA-MB-231.
Compound Libraries for Lin28 1632
Lin28 1632 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Epigenetics Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for Lin28 1632
| M. Wt | 281.31 |
| Formula | C15H15N5O |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 108825-65-6 |
| PubChem ID | 2737312 |
| InChI Key | WPAQLESUVYGUJZ-UHFFFAOYSA-N |
| Smiles | CN(C1=CC=CC(C2=NN3C(C)=NN=C3C=C2)=C1)C(C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Lin28 1632
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.13 | 100 | |
| ethanol | 28.13 | 100 |
Preparing Stock Solutions for Lin28 1632
The following data is based on the product molecular weight 281.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.55 mL | 17.77 mL | 35.55 mL |
| 5 mM | 0.71 mL | 3.55 mL | 7.11 mL |
| 10 mM | 0.36 mL | 1.78 mL | 3.55 mL |
| 50 mM | 0.07 mL | 0.36 mL | 0.71 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Lin28 1632
References for Lin28 1632
References are publications that support the biological activity of the product.
Roos et al (2016) A small-molecule inhibitor of Lin28. ACS Chem.Biol. 11 2773 PMID: 27548809
Chen et al (2019) LIN28/ let-7/PD-L1 pathway as a target for cancer immunotherapy. Cancer Immunol.Res. 7 487 PMID: 30651289
Chen et al (2019) Targeting LIN28B reprograms tumor glucose metabolism and acidic microenvironment to suppress cancer stemness and metastasis. Oncogene 38 4527 PMID: 30742065
If you know of a relevant reference for Lin28 1632, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Lin28 1632, Lin28 1632 supplier, Lin281632, RNA-binding, protein, Lin28, stem, cell, differentiation, bromodomains, inhibitors, inhibits, PD-L1, let-7, antitumor, immunity, Bromodomains, DNA,, RNA, and, Protein, Synthesis, ESCs, iPSC, Immune, Checkpoints, 6068, Tocris Bioscience
3 Citations for Lin28 1632
Citations are publications that use Tocris products. Selected citations for Lin28 1632 include:
Alfredo et al (2020) Functional Characterization of the Lin28/let-7 Circuit During Forelimb Regeneration in Ambystoma mexicanum and Its Influence on Metabolic Reprogramming. Front Cell Dev Biol 8 562940 PMID: 33330447
Miguel N et al (2020) LIN28B Underlies the Pathogenesis of a Subclass of Ewing Sarcoma LIN28B Control of EWS-FLI1 Stability. Cell Rep 30 4567-4583.e5 PMID: 32234488
Yoji et al (2021) Lin28A/let-7 oncogenic circuit is a potential therapeutic target in neurocutaneous melanosis-associated CNS tumors in children. Neurooncol Adv 3 vdaa174 PMID: 33506207
Do you know of a great paper that uses Lin28 1632 from Tocris? Please let us know.
Reviews for Lin28 1632
Average Rating: 5 (Based on 1 Review.)
Have you used Lin28 1632?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Good inhibitor for Lin28.
By
Satbir Thakur on 05/17/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: YP-MEL, T47D and BT183
Used as an inhibitor for Lin28 for 96 h in cell lines expressing Lin28. The protein level was then estimated using a western blot method.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Cell Cycle and DNA Damage Research Product Guide
This product guide provides a review of the cell cycle and DNA damage research area and lists over 150 products, including research tools for:
- Cell Cycle and Mitosis
- DNA Damage Repair
- Targeted Protein Degradation
- Ubiquitin Proteasome Pathway
- Chemotherapy Targets
Huntington's Disease Research Product Guide
This product guide provides a background to Huntington's disease research and lists around 100 products for the study of:
- Somatic Instability
- Proteolysis and Inclusion Bodies
- Transcriptional Dysregulation
- Mitochondrial Dysfunction
- Nuclear-Cytoplasmic Transport Interference
- Excitotoxicity
- Stem Cells