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Submit ReviewML 7 hydrochloride
Description: Selective inhibitor of myosin light chain kinase
Chemical Name: Hexahydro-1-[(5-iodo-1-naphthalenyl)sulfonyl]-1H-1,4-diazepine hydrochloride
Purity: ≥98% (HPLC)
Biological Activity for ML 7 hydrochloride
ML 7 hydrochloride is a selective MLCK inhibitor (Ki = 0.3 μM). Exhibits more potent inhibition than ML 9 hydrochloride (Cat. No. 0431) Displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. Inhibits proplatelet formation and stabilization.
Technical Data for ML 7 hydrochloride
| M. Wt | 452.74 |
| Formula | C15H17IN2O2S.HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 110448-33-4 |
| PubChem ID | 9803932 |
| InChI Key | KDDALCDYHZIZMH-UHFFFAOYSA-N |
| Smiles | IC1=C(C=CC=C2S(N3CCCNCC3)(=O)=O)C2=CC=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ML 7 hydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.64 | 50 |
Preparing Stock Solutions for ML 7 hydrochloride
The following data is based on the product molecular weight 452.74. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.42 mL | 22.09 mL | 44.18 mL |
| 2.5 mM | 0.88 mL | 4.42 mL | 8.84 mL |
| 5 mM | 0.44 mL | 2.21 mL | 4.42 mL |
| 25 mM | 0.09 mL | 0.44 mL | 0.88 mL |
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Product Datasheets for ML 7 hydrochloride
References for ML 7 hydrochloride
References are publications that support the biological activity of the product.
Saitoh et al (1987) Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase. J.Biol.Chem. 262 7796 PMID: 3108259
Meinders et al (2015) Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function. Blood 125 1957 PMID: 25538045
If you know of a relevant reference for ML 7 hydrochloride, please let us know.
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Keywords: ML 7 hydrochloride, ML 7 hydrochloride supplier, ML7, HCl, MLC, kinase, inhibitor, inhibits, myosin, light, chain, inhibitors, MLCKs, platelets, Myosin, Light, Chain, Kinase, 4310, Tocris Bioscience
11 Citations for ML 7 hydrochloride
Citations are publications that use Tocris products. Selected citations for ML 7 hydrochloride include:
Lee et al (2019) Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments. Nat Commun 10 529 PMID: 30705265
Chaudhuri et al (2015) Substrate stress relaxation regulates cell spreading. J Neurosci 6 6364 PMID: 25695512
Bharadwaj (2017) αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun 8 14348 PMID: 28128308
Leoni et al (2015) Annexin A1-containing extracellular vesicles and polymeric nanoparticles promote epithelial wound repair. J Clin Invest 125 1215 PMID: 25664854
Siddoway et al (2013) An essential role for inhibitor-2 regulation of protein phosphatase-1 in synaptic scaling. Am J Physiol Renal Physiol 33 11206 PMID: 23825423
Nicoletta et al (2018) Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell 172 1063-1078.e19 PMID: 29474907
Robert D et al (2014) Eph-Ephrin signaling and focal adhesion kinase regulate actomyosin-dependent apical constriction of ciliary band cells. Development 141 1075-84 PMID: 24550115
William M et al (2022) Synaptopodin stress fiber and contractomere at the epithelial junction. J Cell Biol 221 PMID: 35416930
Clifford J et al (2019) Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development. Cell 179 90-105.e21 PMID: 31539501
Tharp et al (2018) Actomyosin-Mediated Tension Orchestrates Uncoupled Respiration in Adipose Tissues. Cell Metab 27 602 PMID: 29514068
Ets (2016) Sustained Contraction in Vascular Smooth Muscle by Activation of L-type Ca2+ Channels Does Not Involve Ca2+ Sensitization or Caldesmon. Front Pharmacol 7 516 PMID: 28082901
Do you know of a great paper that uses ML 7 hydrochloride from Tocris? Please let us know.
Reviews for ML 7 hydrochloride
Average Rating: 4.5 (Based on 2 Reviews.)
Have you used ML 7 hydrochloride?
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Works well.
By
Wen Ding on 08/04/2020
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: aorta
Mouse from all two groups were housed in condition of a 12 -h shift of light–dark cycle. Mouse of the control group were fed a normal diet, the ML7 group was fed orally treated with ML7 (1 mg/kg daily)
ML7 is good for polarization.
By
Anonymous on 09/12/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: primary MEO
Bright-field (top) images for tetON-Snai1 MEOs treated with doxycycline and 10 μM ML7 for four days.
