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Submit ReviewNADA-green
Description: Fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: 3-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-alanine hydrochloride
Purity: ≥95% (HPLC)
Biological Activity for NADA-green
NADA-green is a fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Results in strong peripheral and septal labeling of taxonomically diverse bacterial cell populations without affecting growth rate. Excitation/emission λ ~450/555 nm.
Please note that the spectral data is solvent dependant
Optical Data for NADA-green
| Emission Color | Green |
|---|---|
| λabs | 450 nm |
| λem | 555 nm |
| Extinction Coefficient (ε) | 25,000 M-1cm-1 |
| Application | Fluorescence Microscopy |
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for NADA-green
| M. Wt | 303.66 |
| Formula | C9H9N5O5.HCl |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 2253733-11-6 |
| PubChem ID | 137919868 |
| InChI Key | NKFVSANWNYYLLB-PGMHMLKASA-N |
| Smiles | N[C@@H](C(O)=O)CNC1=CC=C([N+]([O-])=O)C2=NON=C12.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NADA-green
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.37 | 100 |
Preparing Stock Solutions for NADA-green
The following data is based on the product molecular weight 303.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.29 mL | 16.47 mL | 32.93 mL |
| 5 mM | 0.66 mL | 3.29 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.29 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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Product Datasheets for NADA-green
References for NADA-green
References are publications that support the biological activity of the product.
Kuru et al (2012) In situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids. Angew.Chem.Int.Ed. 51 12519 PMID: 23055266
Kuru et al (2014) Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat.Protoc. 10 33 PMID: 25474031
Ladokhin et al (2002) Determining the Membrane Topology of Proteins: Insertion Pathway of a Transmembrane Helix of Annexin 12 Biochemistry 41 13617 PMID: 12427023
If you know of a relevant reference for NADA-green, please let us know.
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Keywords: NADA-green, NADA-green supplier, fluorescent, green, D-amino, acid, FDAA, peptidoglycans, labeling, bacteria, labels, labelling, Unnatural, Amino, Acids, Fluorescent, Probes, for, Imaging, Bacteria, 6648, Tocris Bioscience
2 Citations for NADA-green
Citations are publications that use Tocris products. Selected citations for NADA-green include:
Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906
Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841
Do you know of a great paper that uses NADA-green from Tocris? Please let us know.
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Literature in this Area
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals