Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewNucleic Acid Dye Green I
Description: High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR
Chemical Name: 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride
Purity: ≥95% (HPLC)
Biological Activity for Nucleic Acid Dye Green I
Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.
This product is sold as a DMSO stock.
Usage Information for Nucleic Acid Dye Green I
- Supplied as a 10,000x concentrate in DMSO.
- Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
- Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
- Use diluted samples within 24 hours to ensure maximal sensitivity.
Optical Data for Nucleic Acid Dye Green I
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 520 nm |
| Application | Real Time PCR, Nucleic Acid Staining, RT-LAMP |
| Alternative For | SYBR® Green I |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Nucleic Acid Dye Green I
| M. Wt | 545.19 |
| Formula | C32H37ClN4S |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 2225748-05-8 |
| PubChem ID | 131742173 |
| InChI Key | IHHRNZWXTFDDLM-UHFFFAOYSA-M |
| Smiles | CCCN(C(N(C1=C/2C=CC=C1)C3=CC=CC=C3)=CC2=C\C4=[N+](C)C5=CC=CC=C5S4)CCCN(C)C.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Nucleic Acid Dye Green I
References for Nucleic Acid Dye Green I
References are publications that support the biological activity of the product.
Karlsen et al (1995) SYBR Green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction. J.Virol.Methods 55 153 PMID: 8576305
Bookout et al (2006) High-throughput real-time quantitative reverse transcription PCR. Curr.Protoc.Mol.Biol. doi: 10.1002/0471142 PMID: 18265376
Lamb et al (2018) Rapid detection of Zika virus in urine samples and infected mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification. Sci.Rep. 8 3803 PMID: 29491389
Lamb et al (2020) Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification PLoS One 15 e0234682 PMID: 32530929
View Related Products by Target
Keywords: Nucleic Acid Dye Green I, Nucleic Acid Dye Green I supplier, High, affinity, double-stranded, DNA, (dsDNA), probe, RT-LAMP, Reverse, Transcription, Loop-Mediated, Isothermal, Amplification, SARS-CoV-2, Zika, RTPCR, RT-PCR, qPCR, q_PCR, Fluorescent, Stains, RNA, Probes, Loop-mediated, and, RT-qPCR, 7246, Tocris Bioscience
Citations for Nucleic Acid Dye Green I
Citations are publications that use Tocris products.
Currently there are no citations for Nucleic Acid Dye Green I.
Reviews for Nucleic Acid Dye Green I
There are currently no reviews for this product. Be the first to review Nucleic Acid Dye Green I and earn rewards!
Have you used Nucleic Acid Dye Green I?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals