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PFI 7

Cat. No. 7960 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Selective GID4 antagonist
Chemical Name: N-((1s,4s)-4-(1H-Benzo[d]imidazol-2-yl)cyclohexyl)-2-(((1H-indol-2-yl)methyl)amino)acetamide dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (3)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for PFI 7

PFI 7 is a selective GID4 antagonist (Kd = 80 nM by SPR assays and EC50 = 0.6 μM by cell-based NanoBRET). PFI 7 binds within the beta -barrel of GID4 substrate binding pocket, disrupting its interaction with the canonical Pro/N-degron peptide in live cells. It alters protein levels of several proteins including RNA helicases as measured by quantitative proteomics.

Licensing Information

This probe is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the PFI 7 probe summary on the SGC website.

Technical Data for PFI 7

M. Wt 474.43
Formula C24H27N5O.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
InChI Key ZDMMYPOICXIDIM-QWLZZUQMSA-N
Smiles O=C(CNCC1=CC2=CC=CC=C2N1)N[C@@H]3CC[C@@H](CC3)C4=NC5=CC=CC=C5N4.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PFI 7

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 47.44 100 with gentle warming
DMSO 47.44 100

Preparing Stock Solutions for PFI 7

The following data is based on the product molecular weight 474.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.11 mL 10.54 mL 21.08 mL
5 mM 0.42 mL 2.11 mL 4.22 mL
10 mM 0.21 mL 1.05 mL 2.11 mL
50 mM 0.04 mL 0.21 mL 0.42 mL

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References for PFI 7

References are publications that support the biological activity of the product.

Owens et al (2024) A chemical probe to modulate human GID4 Pro/N-degron interactions. Nat.Chem.Biol. 20 1164 PMID: 38773330

If you know of a relevant reference for PFI 7, please let us know.

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Keywords: PFI 7, PFI 7 supplier, PFI7, antagonism, antagonists, antagonist, Ubiquitin, E3, Ligases, Pro/N, degron, selective, 7960, Tocris Bioscience

Citations for PFI 7

Citations are publications that use Tocris products.

Currently there are no citations for PFI 7. Do you know of a great paper that uses PFI 7 from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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