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Submit ReviewPonceau S Staining Solution
Description: Stain used to detect protein bands after Western blotting
Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt
Biological Activity for Ponceau S Staining Solution
Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting.
Technical Data for Ponceau S Staining Solution
| M. Wt | 760.57 |
| Formula | C22H12N4Na4O13S4 |
| Storage | Store at RT |
| CAS Number | 6226-79-5 |
| PubChem ID | 101933731 |
| InChI Key | KQHKSGRIBYJYFX-UHFFFAOYSA-J |
| Smiles | OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Ponceau S Staining Solution
| Solubility | Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution) |
Product Datasheets for Ponceau S Staining Solution
References for Ponceau S Staining Solution
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1 Citation for Ponceau S Staining Solution
Citations are publications that use Tocris products. Selected citations for Ponceau S Staining Solution include:
Ronald L et al (2023) Early Mitochondrial Defects in the 5xFAD Mouse Model of Alzheimer's Disease. J Alzheimers Dis 91 1323-1338 PMID: 36617782
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Reviews for Ponceau S Staining Solution
Average Rating: 4.3 (Based on 3 Reviews.)
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Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation..
By
Anonymous on 12/23/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
Protein samples were resolved in NuPAGE 4–12% Bis-Tris gel (Invitrogen) and transferred to 0.2 µm PVDF membrane. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1).
The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection.
Best staining for tracking protein transfer in western blot.
By
Luiz Alexandre Magno on 07/03/2018
Assay Type: In Vitro
Species: Mouse
We use Ponceau S (Cat. No. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). This enables one to incubate the different membrane sections with various primary antibodies.
1. To get a clear background, wash the membrane quickly in PBS after staining2. Ponceau red stain is not stable and will disappear if you destain longer.3. It is advisable to mark with a pencil the position of the bands of interest if you want to cut a membrane in various sections
Western blot confirmation.
By
Anonymous on 01/05/2018
Assay Type: In Vitro
Species: Human
Western blot confirmation
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