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Description: Orange-red TAMRA-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Alternative Names: TADA
Purity: ≥98% (HPLC)
Biological Activity for RADA
RADA is an orange-red TAMRA-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Suitable for prolonged exposure due to red-shift photophysical properties. Suitable for dSTORM and reSTORM. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~554/580 nm.
This product is supplied as a TFA salt, the stoichiometry of which is batch dependant. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Scientific Data
Fluorescence Imaging
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Application of RADA. Using FDAAs RADA and HADA (Catalog # 6647) to study peptidoglycan enzyme and substrate distribution, and the role of polar cell growth and peptidoglycan segregation over time. Image shows peptidoglycan segregation over time. Left to right - 2 mins HADA pulse (cyan), 45 min outgrowth, followed by 2 min RADA chase (magenta) in WT Msm cells. New cell wall (magenta), oldest cell wall (cyan). Scale: 5 μm.
For more information on this work and to request full protocols please read García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 10 e37243 PMID: 30198841
Optical Data for RADA
| Emission Color | Orange |
|---|---|
| λabs | 554 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95000 M-1cm-1 |
| Quantum Yield (φ) | 0.69 |
| Application | dSTORM, reSTORM |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for RADA
| M. Wt | 516.55 |
| Formula | C28H28N4O6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| InChI Key | SMFYRQAHWDWBTD-AVHJOICASA-N |
| Smiles | N[C@@H](C(O)=O)CNC(C1=CC(C(C2=C(C=C(N(C)C)C=C2)O3)=C4C3=C/C(C=C4)=[N+](C)\C)=C(C=C1)C([O-])=O)=O.N[C@@H](C(O)=O)CNC(C5=CC(C([O-])=O)=C(C(C6=C(C=C(N(C)C)C=C6)O7)=C8C7=C/C(C=C8)=[N+](C)\C)C=C5)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for RADA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 51.66 | 100 |
Preparing Stock Solutions for RADA
The following data is based on the product molecular weight 516.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.94 mL | 9.68 mL | 19.36 mL |
| 5 mM | 0.39 mL | 1.94 mL | 3.87 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.94 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
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Product Datasheets for RADA
References for RADA
References are publications that support the biological activity of the product.
Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665
García-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife 7 pii: e37243. PMID: 30198841
Fenton et al (2017) Erratum: CozE is a member of the MreCD complex that directs cell elongation in Streptococcus pneumoniae. Nat.Microbiol. 2 17011 PMID: 28112724
If you know of a relevant reference for RADA, please let us know.
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Keywords: RADA, RADA supplier, Fluorescent, D-amino, acid, FDAA, TADA, TAMRA, labeling, peptidoglycans, bacteria, dSTORM, reSTORM, SRM, super, resolution, microscopy, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6649, Tocris Bioscience
2 Citations for RADA
Citations are publications that use Tocris products. Selected citations for RADA include:
Garcia-Heredia et al (2018) Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. ELife 7 PMID: 30198841
Baranowski et al (2018) Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape. ELife 7 PMID: 30324906
Do you know of a great paper that uses RADA from Tocris? Please let us know.
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Fluorescent Dyes and Probes Research Product Guide
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