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TRIS base

Cat. No. 3163 2 Reviews Submit a Review Datasheet / COA / SDS
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Description: Commonly used laboratory reagent; used to make TBS, TAE and TBE buffers
Chemical Name: 2-Amino-2-(hydroxymethyl)-1,3-propanediol
Purity: ≥99.5%
Datasheet
Citations
Reviews (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for TRIS base

TRIS base is a commonly used laboratory reagent. Highly water soluble. Working pH range 7 - 9. Used in buffer solutions such as Tris-buffered saline (TBS), Tris-acetate-EDTA (TAE) in combination with acetic acid and EDTA (Cat. No. 2811) and Tris-borate-EDTA in combination with boric acid and EDTA.

Technical Data for TRIS base

M. Wt 121.14
Formula C4H11NO3
Storage Store at RT
Purity ≥99.5%
CAS Number 77-86-1
PubChem ID 6503
InChI Key LENZDBCJOHFCAS-UHFFFAOYSA-N
Smiles OCC(CO)(N)CO

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for TRIS base

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 363.42 3000

Preparing Stock Solutions for TRIS base

The following data is based on the product molecular weight 121.14. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
30 mM 0.28 mL 1.38 mL 2.75 mL
150 mM 0.06 mL 0.28 mL 0.55 mL
300 mM 0.03 mL 0.14 mL 0.28 mL
1500 mM 0.01 mL 0.03 mL 0.06 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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Product Datasheets for TRIS base

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for TRIS base

References are publications that support the biological activity of the product.

If you know of a relevant reference for TRIS base, please let us know.

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Keywords: TRIS base, TRIS base supplier, Commonly, used, laboratory, reagent, buffer, TBS, tris, buffered, saline, TAE, TBA, acetate, EDTA, borate, Buffers,, Solvents, and, Solutions, 3163, Tocris Bioscience

Citations for TRIS base

Citations are publications that use Tocris products.

Currently there are no citations for TRIS base. Do you know of a great paper that uses TRIS base from Tocris? Please let us know.

Reviews for TRIS base

Average Rating: 5 (Based on 2 Reviews.)

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Western blot.
By Anonymous on 07/28/2018
Assay Type: In Vitro
Species: Other

We have used this product to make western blot gels of different percentages of acrylamide

Works as expected.
By Anonymous on 01/04/2018
Species: Human

I used this for making TBS buffers for western blots. All seemed to work fine.