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Description: Green-yellow lucifer yellow-based fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Chemical Name: (αR)-α,6-Diamino-1,3-dioxo-5,8-disulfo-1H-benz[de]isoquinoline-2(3H)-propanoic acid
Purity: ≥95% (HPLC)
Biological Activity for YADA
YADA is a green-yellow lucifer yellow-based fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. Incorporated into bacterial cell walls during synthesis. Exhibits large stokes shift and wider emission spectra than other FDAAs, allowing excitation via a violet light source and detection with a green filter. Displays good water solubility, photostability and thermostability. Excitation/emission λ ~426/535 nm.
Optical Data for YADA
| Emission Color | Green-yellow |
|---|---|
| λabs | 426 nm |
| λem | 535 nm |
| Extinction Coefficient (ε) | 24200 M-1cm-1 |
| Quantum Yield (φ) | 0.2 |
| Application | Confocal Microscopy, Super Resolution Microscopy |
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Technical Data for YADA
| M. Wt | 459.41 |
| Formula | C15H13N3O10S2 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1471982-33-8 |
| PubChem ID | 134159307 |
| InChI Key | YXWYHJBYHLJEIR-UHFFFAOYSA-N |
| Smiles | O=C(N(C[C@@H](N)C(O)=O)C1=O)C2=C(C1=CC(S(=O)(O)=O)=C3N)C3=CC(S(=O)(O)=O)=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for YADA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.94 | 100 |
Preparing Stock Solutions for YADA
The following data is based on the product molecular weight 459.41. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.18 mL | 10.88 mL | 21.77 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.35 mL |
| 10 mM | 0.22 mL | 1.09 mL | 2.18 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
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Product Datasheets for YADA
References for YADA
References are publications that support the biological activity of the product.
Hsu et al (2017) Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem.Sci. 8 6313 PMID: 28989665
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Keywords: YADA, YADA supplier, Fluorescent, D-amino, acid, FDAA, labeling, peptidoglycans, bacteria, Probes, for, Imaging, Bacteria, Unnatural, Amino, Acids, 6650, Tocris Bioscience
Citations for YADA
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals