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说明： Unnatural amino acid for inducing red shift in fluorescent proteins and fluorescent protein-based biosensors
化学名： 3-Amino-L-tyrosine dihydrochloride
纯度： ≥98% (HPLC)
生物活性 for 3-Aminotyrosine
3-Aminotyrosine is an unnatural amino acid that induces red shift when incorporated into fluorescent proteins and fluorescent protein-based biosensors. Causes spontaneous and efficient red shift of GFP in HEK293T cells, shifting excitation and emission maxima by 56 and 95 nm respectively. Induces red shift in a range of fluorescent proteins containing tyrosine-derived chromophores, including teal fluorescent mTFP1, yellow fluorescent Citrine, and circularly permuted YFP and GFP. Also induces red-shift in fluorescent protein-based biosensors, including iGluSnFR (a glutamate sensor), iGABASnFR (a GABA sensor), dLight1.2 (a dopamine sensor) and iATPSnFR1.1 (an ATP sensor).
技术数据 for 3-Aminotyrosine
| 分子量 | 269.12 |
| 公式 | C9H12N2O3.2HCl |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 23279-22-3 |
| PubChem ID | 46735196 |
| InChI Key | PIFWBMTWCTUOOQ-KLXURFKVSA-N |
| Smiles | N[C@H](C(O)=O)CC1=CC(N)=C(C=C1)O.Cl.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for 3-Aminotyrosine
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 26.91 | 100 | |
| DMSO | 26.91 | 100 |
制备储备液 for 3-Aminotyrosine
以下数据基于产品分子量 269.12。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.72 mL | 18.58 mL | 37.16 mL |
| 5 mM | 0.74 mL | 3.72 mL | 7.43 mL |
| 10 mM | 0.37 mL | 1.86 mL | 3.72 mL |
| 50 mM | 0.07 mL | 0.37 mL | 0.74 mL |
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产品说明书 for 3-Aminotyrosine
参考文献 for 3-Aminotyrosine
参考文献是支持产品生物活性的出版物。
Zhang & Ai (2020) A general strategy to red-shift green fluorescent protein-based biosensors. Nat.Chem.Biol. 16 1434 PMID: 32929278
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关键词: 3-Aminotyrosine, 3-Aminotyrosine supplier, red, shift, fluorescent, protein, based, biosensors, GFP, YFP, TFP, noncanonical, unnatural, amino, acids, UAA, Unnatural, Amino, Acids, 7370, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
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