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Submit ReviewKey information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.
Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.
Emission Color | Blue |
---|---|
λabs | 404 nm |
λem | 477 nm |
Application | Fluorescence Microscopy |
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 203.15 |
公式 | C9H5N3O3 |
储存 | Store at -20°C |
纯度 | ≥95% (HPLC) |
CAS Number | 817638-68-9 |
PubChem ID | 25127608 |
InChI Key | KKJNQKVCZCNJDI-UHFFFAOYSA-N |
Smiles | [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 20.32 | 100 | |
DMF | 20.32 | 100 |
以下数据基于产品分子量 203.15。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 4.92 mL | 24.61 mL | 49.22 mL |
5 mM | 0.98 mL | 4.92 mL | 9.84 mL |
10 mM | 0.49 mL | 2.46 mL | 4.92 mL |
50 mM | 0.1 mL | 0.49 mL | 0.98 mL |
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关键词: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience
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