3-Azido-7-hydroxycoumarin

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说明: Click-reactive fluorescent dye. Suitable for live cell imaging
化学名: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
纯度: ≥95% (HPLC)
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生物活性 for 3-Azido-7-hydroxycoumarin

Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.

Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.

Optical Data for 3-Azido-7-hydroxycoumarin

Emission Color Blue
λabs 404 nm
λem 477 nm
Application Fluorescence Microscopy

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技术数据 for 3-Azido-7-hydroxycoumarin

分子量 203.15
公式 C9H5N3O3
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 817638-68-9
PubChem ID 25127608
InChI Key KKJNQKVCZCNJDI-UHFFFAOYSA-N
Smiles [N-]=[N+]=NC1=CC=2C=CC(O)=CC2OC1=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for 3-Azido-7-hydroxycoumarin

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 20.32 100
DMF 20.32 100

制备储备液 for 3-Azido-7-hydroxycoumarin

以下数据基于产品分子量 203.15。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 4.92 mL 24.61 mL 49.22 mL
5 mM 0.98 mL 4.92 mL 9.84 mL
10 mM 0.49 mL 2.46 mL 4.92 mL
50 mM 0.1 mL 0.49 mL 0.98 mL

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产品说明书 for 3-Azido-7-hydroxycoumarin

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关键词: 3-Azido-7-hydroxycoumarin, 3-Azido-7-hydroxycoumarin supplier, 3azido7hydroxycoumarin, fluorescence, triazoles, Fluorescent, fluorogenic, dyes, dye, imaging, Click, Reactive, Click-Reactive, alkyne, Dyes, 7664, Tocris Bioscience

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