5(6)-SFX (Fluorescein), SE

Name: 5(6)-SFX (Fluorescein), SE
Brand: Tocris Bioscience
SKU: 6488

Cat. No. 6488 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Discontinued Product

6488 has been discontinued.

View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.

说明： Green fluorescent dye for the labeling of primary amines

化学名： 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

纯度： ≥90% (HPLC)

说明书

引用文献

实验方案 (1)

文献 (1)

生物活性技术数据说明书参考文献

生物活性 for 5(6)-SFX (Fluorescein), SE

5(6)-SFX (Fluorescein), SE is a green fluorescent dye for the labeling of primary amines. Comprising fluorescein, with a commonly used fluorophore, the hexanoic spacer that separates the fluorophore from the molecule to which it is conjugated and makes the NHS ester group more accessible to biopolymers for conjugation. Excitation maximum = 494 nm; emission maximum = 520 nm, extinction coefficient: 74,000 cm^-1M^-1.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Optical Data for 5(6)-SFX (Fluorescein), SE

Emission Color	Green
λ_abs	494 nm
λ_em	518 nm
Extinction Coefficient (ε)	70000 M^-1cm^-1
Closest Laser line	488 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Application	Fluorescence Microscopy, Flow Cytometry, Immunofluorescence Assays (i.e. Western Blot, ELISA, etc)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for 5(6)-SFX (Fluorescein), SE

分子量	586.55
公式	C₃₁H₂₆N₂O₁₀
储存	Store at -20°C
纯度	≥90% (HPLC)
CAS Number	114616-31-8
PubChem ID	122174028
InChI Key	WAGYELYAQOYZFU-UHFFFAOYSA-N
Smiles	OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)OC(C5=C3C=CC(C(NCCCCCC(ON6C(CCC6=O)=O)=O)=O)=C5)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)OC(C%11=C9C=C(C(NCCCCCC(ON%12C(CCC%12=O)=O)=O)=O)C=C%11)=O)C=C7

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for 5(6)-SFX (Fluorescein), SE

分析证书/产品说明书

化学品安全技术说明书

参考文献 for 5(6)-SFX (Fluorescein), SE

参考文献是支持产品生物活性的出版物。

Ito et al (2015) Artificial human Met agonists based on macrocycle scaffolds. Nat.Commun. 6 6373 PMID: 25758345

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