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Discontinued Product
7665 has been discontinued.
View all Standard Fluorescein, Coumarin and Rhodamine Dyes products.
说明： Fluorescent amine-reactive dye for labeling proteins, peptides and oligonucleotides, FRET acceptor for FAM
化学名： 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-5-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
纯度： ≥90% (HPLC)
生物活性 for 5-TAMRA NHS
Key information: 5-TAMRA NHS is an orange fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Can be used for oligonucleotide labeling.
Application: Suitable for fluorescence microscopy, confocal microscopy, FRET acceptor for FAM.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 546 nm and 580 nm, respectively; quantum yield = 0.1; extinction coefficient = 95,000 M-1cm-1; A280 = 0.178.
Please see the protocol for further information on protein/antibody labeling and conjugation.
It is recommended to prepare stock solutions in DMSO.
Optical Data for 5-TAMRA NHS
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 546 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 95,000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| pH Sensitivity | No |
| Correction Factor 280 | 0.178 |
| Application | Fluorescence Microscopy, Confocal Microscopy |
| Alternative For | Alexa Fluor® 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for 5-TAMRA NHS
| 分子量 | 527.52 |
| 公式 | C29H25N3O7 |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 150810-68-7 |
| PubChem ID | 2762600 |
| InChI Key | VWFRSNKRTNUMET-UHFFFAOYSA-N |
| Smiles | CN(C)C1=CC=C(C(C2=CC=C(C(ON3C(CCC3=O)=O)=O)C=C2C([O-])=O)=C(C=C/4)C(O5)=CC4=[N+](C)\C)C5=C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for 5-TAMRA NHS
参考文献 for 5-TAMRA NHS
参考文献是支持产品生物活性的出版物。
Kvach et al (2009) Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes. Bioconjug.Chem. 20 1673 PMID: 19606815
Hirata et al (2014) Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres. J.Am.Chem.Soc. 136 11546 PMID: 25036716
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关键词: 5-TAMRA, NHS, 5-TAMRA, NHS supplier, 5TAMRANHS, 5tamrase, fluorescent, dyes, DNA, RNA, amine, reactive, peptides, oligonucleotides, proteins, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, 7665, Tocris Bioscience
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该领域的文献
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals