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Submit ReviewArtificial cerebrospinal fluid (ACSF) is commonly used to maintain the oxygen supply, osmolarity and buffer pH of isolated neurons and brain slices in electrophysiology experiments. ACSF closely matches the electrolyte concentrations of cerebrospinal fluid and is prepared from high purity water and analytical grade reagents. Microfiltered and sterile. Final ion concentrations (in mM): Na+ 150; K+ 3.0; Ca2+ 1.4; Mg2+ 0.8; P 1.0; Cl- 155.
储存 | Store at RT |
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Tocris products are intended for laboratory research use only, unless stated otherwise.
参考文献是支持产品生物活性的出版物。
Byun et al (2012) Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease. PLoS One 7 e37917 PMID: 22662249
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关键词: ACSF (Artificial Cerebrospinal Fluid), ACSF (Artificial Cerebrospinal Fluid) supplier, Artificial, cerebrospinal, fluid, Reagents, CSF, electrophysiology, pH, buffer, ACSF, cerebro, spinal, Biochemicals, and, Molecular, Biology, 3525, Tocris Bioscience
引用文献是使用了 Tocris 产品的出版物。 ACSF (Artificial Cerebrospinal Fluid) 的部分引用包括:
Bake et al (2017) Acute ghrelin changes food preference from a high-fat diet to chow during binge-like eating in rodents. J Neuroendocrinol 29 PMID: 28219000
Maurin et al (2014) Hypothalamic eIF2α signaling regulates food intake. Cell Rep 6 438 PMID: 24485657
Mitroi et al (2017) SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production. Autophagy 13 885 PMID: 28521611
Rau et al (2015) Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress. Nat Commun 35 9730 PMID: 26134655
Suberbielle et al (2015) DNA repair factor BRCA1 depletion occurs in Alzheimer brains and impairs cognitive function in mice. Front Pharmacol 6 8897 PMID: 26615780
Feetham et al (2015) The depressor response to intracerebroventricular hypotonic saline is sensitive to TRPV4 antagonist RN1734. Electrophoresis 6 83 PMID: 25954200
Yoo et al (2019) Tanycyte-Independent Control of Hypothalamic Leptin Signaling. Front Neurosci 13 240 PMID: 30941008
Norwood et al (2019) Anatomical basis and physiological role of cerebrospinal fluid transport through the murine cribriform plate. Elife 8 PMID: 31063132
Lam et al (2016) Absence of systemic oxidative stress and increased CSF prostaglandin F2α in progressive MS Neurology: Neuroimmunology & Neuroinflammation 3 256 PMID: 27386506
Desmoulins et al (2019) Mitochondrial Dynamin-Related Protein 1 (DRP1) translocation in response to cerebral glucose is impaired in a rat model of early alteration in hypothalamic glucose sensing. Mol Metab 20 166 PMID: 30553770
Taylor et al (2014) Investigating local and long-range neuronal network dynamics by simultaneous optogenetics, reverse microdialysis and silicon probe recordings in vivo. J Neurosci 235 83 PMID: 25004203
Handa et al (2013) Central 5-α reduction of testost. is required for testosterone's inhibition of the hypothalamo-pituitary-adrenal axis response to restraint stress in adult male rats. J Neurosci Methods 1529 74 PMID: 23880372
Bake et al (2018) Ghrelin's effects on food motivation in rats are not limited to palatable foods. J Neuroendocrinol e12665 PMID: 30525248
Sartor et al (2015) Epigenetic Readers of Lysine Acetylation Regulate Cocaine-Induced Plasticity. J Neurosci 35 15062 PMID: 26558777
Phillips and Wellner (2013) Detection of cerebral spinal fluid-associated chemokines in birth traumatized premature babies by chip-based immunoaffinity CE. J Biol Chem 34 1530 PMID: 23512340
McCall et al (2013) Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics. Nat Protoc 8 2413 PMID: 24202555
Miled & Sawan (2012) Electrode robustness in artificial cerebrospinal fluid for dielectrophoresis-based LoC. Conf Proc IEEE Eng Med Biol Soc 2012 1390 PMID: 23366159
Hill et al (2018) Activation of GPR55 increases neural stem cell proliferation and promotes early adult hippocampal neurogenesis. Br.J.Pharmacol. 175 3407 PMID: 29888782
Claflin et al (2017) Angiotensin AT1A receptors on leptin receptor-expressing cells control resting metabolism. J Clin Invest 127 1414 PMID: 28263184
Zhang et al (2017) Astrocytic Process Plasticity and IKKβ/NF-κB in Central Control of Blood Glucose, Blood Pressure, and Body Weight. Cell Metab 25 1091 PMID: 28467927
Ramírez et al (2017) Mitochondrial Dynamics Mediated by Mitofusin 1 Is Required for POMC Neuron Glucose-Sensing and ins. Release Control. Cell Metab 25 1390 PMID: 28591639
Kurland et al (2016) The Sur1-Trpm4 channel regulates NOS2 transcription in TLR4-activated microglia. J Neuroinflammation 13 130 PMID: 27246103
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We use aCSF to infuse drugs into the brain. We usually dissolve the different drugs that we infuse in aCSF and then use aCSF infusions as a control