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Submit ReviewBDY FL VH032
说明： High-affinity VHL fluorescent probe for TR-FRET and FP assays
化学名： (2S,4R)-1-((S)-2-(tert-Butyl)-21-(5,5-difluoro-7,9-dimethyl-5H-5λ4,6λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-4,19-dioxo-6,9,12,15-tetraoxa-3,18-diazahenicosanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide
纯度： ≥95% (HPLC)
生物活性 for BDY FL VH032
BDY FL VH032 is a high-affinity VHL fluorescent probe (Kd = 3.01 nM) with application in time-resolved fluorescence resonance energy-transfer (TR-FRET) assays for high-throughput identification and characterization of VHL ligands. BDY FL VH032 consists of a derivative of von Hippel-Lindau (VHL) ligand VH 032 (Cat. No. 6911) and a fluorophore BDY FL (Cat. No. 5465) (with excitation peak at 504 nm and emission peak at 520 nm), joined by a polyethylene glycol (PEG) 4 linker. The BDY FL VH032 VHL TR-FRET signal is stable through the 90 - 300 min incubation time, and the TR-FRET binding assay is sensitive, selective and resistant to assay interference. BDY FL VH032 also exhibits high VHL affinity in a fluorescence polarization (FP) assay. Excitation maximum ~ 504 nm; emission maximum ~ 520 nm.
许可信息
Sold under license from St. Jude Children's Research Hospital
Optical Data for BDY FL VH032
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 502 nm |
| λem | 510 nm |
| Closest Laser line | 488 nm |
| Application | TR-FRET and FP Assays |
| Alternative For | FAM-DEALAHypYIPMDDDFQLRSF, FAM-DEALA-Hyp-YIPD |
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技术数据 for BDY FL VH032
| 分子量 | 937.91 |
| 公式 | C46H62BF2N7O9S |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 2770675-66-4 |
| InChI Key | RYFBKCMIPSQUPY-UOIAJAMCSA-N |
| Smiles | O=C(N[C@@H](C(C)(C)C)C(N1C[C@H](O)C[C@H]1C(NCC2=CC=C(C3=C(C)N=CS3)C=C2)=O)=O)COCCOCCOCCOCCNC(CCC4=CC=C5N4[B-]([N+]6=C(C)C=C(C)C6=C5)(F)F)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for BDY FL VH032
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 9.38 | 10 |
制备储备液 for BDY FL VH032
以下数据基于产品分子量 937.91。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 10.66 mL | 53.31 mL | 106.62 mL |
| 0.5 mM | 2.13 mL | 10.66 mL | 21.32 mL |
| 1 mM | 1.07 mL | 5.33 mL | 10.66 mL |
| 5 mM | 0.21 mL | 1.07 mL | 2.13 mL |
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产品说明书 for BDY FL VH032
参考文献 for BDY FL VH032
参考文献是支持产品生物活性的出版物。
Lin et al (2020) Development of BODIPY FL VH032 as a high-affinity and selective von Hippel-Lindau E3 ligase. ACS Omega 6 680 PMID: 33458521
If you know of a relevant reference for BDY FL VH032, please let us know.
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关键词: BDY FL VH032, BDY FL VH032 supplier, selective, high-affinity, VHL, E3, ligase, fluorescent, probe, BODIPY, FL, VH032, TR-FRET, fluorescence, polarization, FP, Enzyme, Probes, and, Substrates, Ubiquitin, Ligases, Assay, Reagents, 7483, Tocris Bioscience
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