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Submit ReviewBiocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.
分子量 | 372.48 |
公式 | C16H28N4O4S |
储存 | Store at -20°C |
CAS Number | 576-19-2 |
PubChem ID | 83814 |
InChI Key | BAQMYDQNMFBZNA-MNXVOIDGSA-N |
Smiles | O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
water | 18.62 | 50 温和加热 |
以下数据基于产品分子量 372.48。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.5 mM | 5.37 mL | 26.85 mL | 53.69 mL |
2.5 mM | 1.07 mL | 5.37 mL | 10.74 mL |
5 mM | 0.54 mL | 2.68 mL | 5.37 mL |
25 mM | 0.11 mL | 0.54 mL | 1.07 mL |
参考文献是支持产品生物活性的出版物。
Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327 PMID: 10856608
Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35 PMID: 15334648
MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6 PMID: 17990268
If you know of a relevant reference for Biocytin, please let us know.
关键词: Biocytin, Biocytin supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Biochemicals, and, Molecular, Biology, 3349, Tocris Bioscience
引用文献是使用了 Tocris 产品的出版物。 Biocytin 的部分引用包括:
Lam et al (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun 13 584 PMID: 21531340
Estakhr et al (2017) Differential Control of Dopaminergic Excitability and Locomotion by Cholinergic Inputs in Mouse Substantia Nigra. Curr Biol 27 1900 PMID: 28648825
Kim and Cho (2017) Encoding of Discriminative Fear Memory by Input-Specific LTP in the Amygdala. Neuron 95 1129 PMID: 28823727
Chai et al (2017) Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence. Neuron 95 531 PMID: 28712653
Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70 PMID: 21904526
Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586 PMID: 25945713
Roesler et al (2019) Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Front Cell Neurosci 13 330 PMID: 31474830
Ferrarese et al (2018) Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Rep 24 3455 PMID: 30257207
Tsai et al (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio 488 647 PMID: 22763451
Xia et al (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One 9 e100968 PMID: 24971996
Cunningham et al (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell 15 559 PMID: 25517465
Dettmann et al (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One 9 e97792 PMID: 24871092
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