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Submit ReviewBovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.
MW | ~66 kDa |
储存 | Store at +4°C |
纯度 | ≥98% |
CAS Number | 9048-46-8 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性 | Soluble to 100 mg/ml in water |
参考文献是支持产品生物活性的出版物。
If you know of a relevant reference for Bovine Serum Albumin, please let us know.
关键词: Bovine Serum Albumin, Bovine Serum Albumin supplier, Bovine, Serum, Albumin, BSA, solution, powder, blocking, buffers, agents, immunohistochemistry, IHC, Immunocytochemistry, ICC, ELISAs, Western, blotting, blots, non-specific, binding, cell, culture, Buffers,, Solvents, and, Solutions, Blocking, Buffer, 5217, Tocris Bioscience
引用文献是使用了 Tocris 产品的出版物。 Bovine Serum Albumin 的部分引用包括:
Pamela J et al (2022) SPG15 protein deficits are at the crossroads between lysosomal abnormalities, altered lipid metabolism and synaptic dysfunction. Hum Mol Genet 31 2693-2710 PMID: 35313342
P Michael et al (2022) The Circadian Clock in the Retinal Pigment Epithelium Controls the Diurnal Rhythm of Phagocytic Activity. Int J Mol Sci 23 PMID: 35628111
George P et al (2020) High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme. Sci Rep 10 22370 PMID: 33353972
David et al (2020) Dopamine 2 Receptor Signaling Controls the Daily Burst in Phagocytic Activity in the Mouse Retinal Pigment Epithelium. Invest Ophthalmol Vis Sci 61 10 PMID: 32396631
Serena et al (2023) Automated high-content imaging in iPSC-derived neuronal progenitors. SLAS Discov 28 42-51 PMID: 36610640
Glover et al (2023) The developmental basis of fingerprint pattern formation and variation Cell 186 1 PMID: 36764291
Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression, and cell proliferation. J Cell Sci PMID: 34031693
Matthias et al (2021) Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation. J Cell Sci 134 PMID: 34152388
Hanne C et al (2018) Comparison of two different culture conditions for derivation of early hiPSC. Cell Biol Int 42 1467-1473 PMID: 29603519
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平均评分: 4.5 (基于 4 条评论。)
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5% blocking buffer for immunoflorescent staining.
5% BSA diluted in PBS. 30 min incubation with blocking buffer at RT
in the tc and western blotting
We use this product for WB. It works very well.
Used to block PVDF membranes for WB. Perfect performance. Works better than milk