Cy5B-trisulfo, NHS

Cat. No. 7394 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Restricted conformation cyanine dye with high quantum yield and long fluorescence lifetime for advanced microscopy applications

实验方案 (1)

文献 (1)

生物活性 Scientific Data 技术数据说明书参考文献

生物活性 for Cy5B-trisulfo, NHS

Key information: Cy5B-trisulfo, NHS is a far-red fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Particularly useful for smFRET experiments to reduce blinking and bleaching artefacts and maintain correct mean FRET efficiencies.

Application: FRET, smFRET, FLIM, TIRF, and super resolution microscopy (SRM) techniques, including dSTORM. Provides enhanced spatial resolution in dSTORM experiments. Does not provide efficient blinking in conventional high-thiol dSTORM buffers; instead, sodium borohydride (NaBH₄) addition is required following which SMLM experiments can be performed in PBS.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 669 nm and 685 nm, respectively; extinction coefficient = 241,000 M-1cm-1; quantum yield = 0.4; fluorescence lifetime = 1.85 ns.

We suggest making up a 10 mM stock solution of this product in anhydrous DMSO.

For more information on how Cy5B-trisulfo, NHS may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Scientific Data

Fluorescence Imaging

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Application of Cy5B-trisulfo, NHS in COS7. FLIM composite image of a COS7 cell immunostained with mouse anti-beta -tubulin primary and Cyanine 5 labelled secondary (green), and rabbit anti-clathrin primary plus Cy5B-trisulfo, NHS labelled secondary (yellow-red). Scale bars, 5 μm and 1 μm (insets). Image kindly provided by Dr. Martin Schnermann, Center for Cancer Research, National Cancer Institute.

Super-Resolution Microscopy

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Application of Cy5B-trisulfo, NHS in microtubules. Single-molecule localization microscopy (SMLM) images obtained using TIRF microscopy of microtubules immunolabeled with Cy5B-trisulfo, NHS labeled anti-tubulin antibody in 0.1% NaBH4/PBS upon irradiation with 640 nm (2.5 kWcm^-2 ) using an integration time of 20 ms (total acquisition time 10 min). Scale bars, 3 μm and 1 μm (insets). Fourier ring correlation (FRC) analysis was used to estimate the indicated spatial resolutions. Image kindly provided by Dr. Martin Schnermann, Center for Cancer Research, National Cancer Institute.

许可信息

Sold under license from the National Institute of Health

Optical Data for Cy5B-trisulfo, NHS

Emission Color	Far Red
λ_abs	669 nm
λ_em	685 nm
Extinction Coefficient (ε)	241,000 M^-1cm^-1
Quantum Yield (φ)	0.4
Closest Laser line	640 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Application	SMLM, FRET, smFRET, FLIM, dSTORM

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for Cy5B-trisulfo, NHS

分子量	1055.11
公式	C₄₇H₅₂N₄Na₂O₁₅S₃
储存	Store at -20°C
InChI Key	WHRLQGVVUOKRRS-IOAHUSCESA-L
Smiles	CC1(C)C2=C(C=CC(S(=O)(O[Na])=O)=C2)N(CC[C@@]3([H])C[C@@]4([H])O[C@@]5([H])CC6)C1=C3C=C4C=C5C7=[N+]6C8=C(C=C(S(=O)([O-])=O)C=C8)C7(CCCCC(N(CCC(ON9C(CCC9=O)=O)=O)CCCS(=O)(O[Na])=O)=O)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for Cy5B-trisulfo, NHS

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Cy5B-trisulfo, NHS

参考文献是支持产品生物活性的出版物。

Eiring et al (2021) Targetable conformationally restricted Cyanines enable photon-count-limited applications. Angew.Chem.Int.Ed.Engl. 60 26685 PMID: 34606673

Wäldchen et al (2020) Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM. Nat.Commun. 11 887 PMID: 32060305

Eiring et al (2021) Targetable conformationally restricted cyanines enable photon-count-limited applications. Angew Chem.Int.Ed.Engl. 60 26685 PMID: 34606673

If you know of a relevant reference for Cy5B-trisulfo, NHS, please let us know.

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