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Submit ReviewCyanine 3, SE
说明： Orange fluorescent dye for labeling of amines; supplied as NHS ester
化学名： 2-[3-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1-propen-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
纯度： ≥95% (HPLC)
生物活性 for Cyanine 3, SE
Cyanine 3, SE is an orange fluorescent dye for labeling of amines. Emission maximum ~564 nm.
Optical Data for Cyanine 3, SE
| Emission Color | Orange |
|---|---|
| Brightness | |
| λabs | 550 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 150000 M-1cm-1 |
| Quantum Yield (φ) | 0.31 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Correction Factor 260 | 0.04 |
| Correction Factor 280 | 0.09 |
| Application | Flow Cytometry |
| Alternative For | Alexa Fluor 546; DyLight 549 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for Cyanine 3, SE
| 分子量 | 727.84 |
| 公式 | C35H41N3O10S2 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 146368-16-3 |
| PubChem ID | 91811030 |
| InChI Key | SOVMUUCAZOFIQP-UHFFFAOYSA-N |
| Smiles | CC[N+]1=C(C(C)(C)C2=C1C=CC(S(=O)([O-])=O)=C2)/C=C/C=C3N(CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC(S(=O)(O)=O)=C5)=C5C/3(C)C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Cyanine 3, SE
参考文献 for Cyanine 3, SE
参考文献是支持产品生物活性的出版物。
Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407
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关键词: Cyanine 3, SE, Cyanine 3, SE supplier, cyanine, NHS, ester, orange, fluorescent, dye, imaging, amines, labeling, Cy3, Cyanine, Dyes, (Cy, Dyes), Flow, Cytometry, 5435, Tocris Bioscience
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Fluorescent probe production and application.
By
Vladimir Khayenko on 03/20/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： Cortical neurons
The Sulfo-Cyanine dye was conjugated to bioactive peptide that binds gephyrin. The labeling of gephyrin was done in Cortical neurons (DIV21) infected with lentivirus to express mEos2-Gephyrin.
该领域的文献
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*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals