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Cyanine 5, SE

Cat. No. 5436 1 次引用 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Red fluorescent dye (Reactive group: NHS ester). Application: confocal microscopy, fluorescence microscopy, flow cytometry
别名： Cy5 NHS Ester
化学名： 2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
纯度： ≥95% (HPLC)
说明书
引用文献 (1)
评论
实验方案 (1)
文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Cyanine 5, SE

Key information: Cyanine 5, SE is a widely used red fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.

Application: Suitable for fluorescence microscopy, confocal microscopy and flow cytometry.

Properties and Photophysical Data: Excitation and emission maxima (λ) are 649 nm and 666 nm, respectively; quantum yield = 0.2; extinction coefficient = 250,000 M-1cm-1; A280 correction factor = 0.04.

For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Optical Data for Cyanine 5, SE

Cyanine 5, SE Dye Spectra
Emission Color Red
Brightness
λabs 649 nm
λem 666 nm
Extinction Coefficient (ε) 250000 M-1cm-1
Quantum Yield (φ) 0.2
Closest Laser line 647 nm
Reactive Group NHS ester
Reactivity Primary amines
Cell Permeable No
Correction Factor 260 0.03
Correction Factor 280 0.04
Application Fluorescence Microscopy, Confocal Microscopy, Flow cytometry
Alternative For Alexa Fluor 647; DyLight 649

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectral Viewer

技术数据 for Cyanine 5, SE

分子量 753.88
公式 C37H43N3O10S2
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 146368-14-1
PubChem ID 118705155
InChI Key WXWLHDCCGVWTDZ-UHFFFAOYSA-N
Smiles CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Cyanine 5, SE

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 75.39 100
DMF 75.39 100

制备储备液 for Cyanine 5, SE

以下数据基于产品分子量 753.88。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 1.33 mL 6.63 mL 13.26 mL
5 mM 0.27 mL 1.33 mL 2.65 mL
10 mM 0.13 mL 0.66 mL 1.33 mL
50 mM 0.03 mL 0.13 mL 0.27 mL

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产品说明书 for Cyanine 5, SE

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for Cyanine 5, SE

参考文献是支持产品生物活性的出版物。

Shin et al (2014) Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 42 e90 PMID: 24753407

If you know of a relevant reference for Cyanine 5, SE, please let us know.

按标靶查看相关产品

关键词: Cyanine 5, SE, Cyanine 5, SE supplier, Cyanine, 5SE, red, fluorescent, dyes, amines, labeling, Cy5, NHS, Ester, Dyes, (Cy, Dyes), Flow, Cytometry, 5436, Tocris Bioscience

1 篇 Cyanine 5, SE 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Cyanine 5, SE 的部分引用包括：

Erik A et al (2022) Directed Evolution of Fluorescent Proteins in Bacteria. Methods Mol Biol 2564 75-97 PMID: 36107338

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Cyanine 5, SE 实验方案

以下实验方案包含使用 Cyanine 5, SE（目录编号 5436）的更多信息。

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Fluorescent Dyes and Probes Research Product Guide

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