Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewDBCO PEG4 Biotin
说明： Biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free click chemistry
化学名： (3aS,4S,6aR)-N-[19-(11,12-Didehydrodibenz[b,f]azocin-5(6H)-yl)-15,19-dioxo-3,6,9,12-tetraoxa-16-azanonadec-1-yl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide
纯度： ≥95% (HPLC)
生物活性 for DBCO PEG4 Biotin
DBCO-PEG4-Biotin is a biotinylation reagent for labeling azide-containing biomolecules via Cu(I)-free Strain-Promoted Alkyne-Azide Click Chemistry (SPAAC) reaction. The hydrophilic PEG4 linker reduces or eliminates aggregation and precipitation during the labeling process by increasing the hydrophilicity of the target molecule. The PEG linker in DBCO-PEG4-Biotin also enhances the accessibility of the biotin moiety, improving the detection efficiency of the biotinylated molecule via fluorescent or HRP-labeled streptavidin or its affinity purification via streptavidin agarose.
技术数据 for DBCO PEG4 Biotin
| 分子量 | 749.92 |
| 公式 | C39H51N5O8S |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 1255942-07-4 |
| PubChem ID | 91757917 |
| InChI Key | LNHSQAOQVNHUGL-QRBHCBQLSA-N |
| Smiles | C(CCCC(NCCOCCOCCOCCOCCC(NCCC(=O)N1C=2C(C#CC=3C(C1)=CC=CC3)=CC=CC2)=O)=O)[C@H]4[C@@]5([C@](CS4)(NC(=O)N5)[H])[H] |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for DBCO PEG4 Biotin
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 74.99 | 100 | |
| ethanol | 74.99 | 100 |
制备储备液 for DBCO PEG4 Biotin
以下数据基于产品分子量 749.92。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.33 mL | 6.67 mL | 13.33 mL |
| 5 mM | 0.27 mL | 1.33 mL | 2.67 mL |
| 10 mM | 0.13 mL | 0.67 mL | 1.33 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for DBCO PEG4 Biotin
参考文献 for DBCO PEG4 Biotin
参考文献是支持产品生物活性的出版物。
Agard et al (2006) A comparative study of bioorthogonal reactions with azides. ACS Chem.Biol. 1 644 PMID: 17175580
Bernardim et al (2020) Precise installation of diazo-tagged side-chains on proteins to enable in vitro and in-cell site-specific labeling. Bioconjug.Chem. 31 1604 PMID: 32375474
Darabedian et al (2020) O-Acetylated chemical reporters of glycosylation can display metabolism-dependent background labeling of proteins but are generally reliable tools for the identification of glycoproteins. Front.Chem. 8 318 PMID: 32411667
Sawant et al (2016) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acids Res. 44 e16 PMID: 26384420
If you know of a relevant reference for DBCO PEG4 Biotin, please let us know.
按标靶查看相关产品
关键词: DBCO PEG4 Biotin, DBCO PEG4 Biotin supplier, Cu(I)-free, Strain-Promoted, Alkyne-Azide, Click, Chemistry, SPAAC, copper-free, biotinylation, labeling, labelling, HRP, horseradish, peroxidase, streptavidin, fluorescent, fluorescence, imaging, Biochemicals, and, Molecular, Biology, 7480, Tocris Bioscience
篇 DBCO PEG4 Biotin 的引用文献
引用文献是使用了 Tocris 产品的出版物。
目前没有 DBCO PEG4 Biotin 的引用文献。 您是否知道使用了 Tocris DBCO PEG4 Biotin 的优秀论文？ 请告知我们.
DBCO PEG4 Biotin 的评论
目前没有该产品的评论。 Be the first to review DBCO PEG4 Biotin and earn rewards!
Have you used DBCO PEG4 Biotin?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image