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DOPE

Cat. No. 7175 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Liposome/lipoplex transfection enhancer
化学名： (9Z)-1,1'-[(1R)-1-[[[(2-Aminoethoxy)hydroxyphosphinyl]oxy]methyl]-1,2-ethanediyl] 9-octadecenoic acid ester
纯度： ≥95% (HPLC)
说明书
引用文献
评论
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for DOPE

DOPE is a liposome/lipoplex transfection reagent. Enhances liposome and lipoplex transfection efficiency in combination with DA, DOTAP or DC-cholesterol (Cat. No. 7176).

Please note: exact ratio for use and transfection efficiency is dependent on cell line used.

技术数据 for DOPE

分子量 744.05
公式 C41H78NO8P
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 4004-05-1
PubChem ID 9546757
InChI Key MWRBNPKJOOWZPW-NYVOMTAGSA-N
Smiles CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](CO[P](O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for DOPE

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
chloroform 74.4 100

制备储备液 for DOPE

以下数据基于产品分子量 744.05。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 1.34 mL 6.72 mL 13.44 mL
5 mM 0.27 mL 1.34 mL 2.69 mL
10 mM 0.13 mL 0.67 mL 1.34 mL
50 mM 0.03 mL 0.13 mL 0.27 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Dilution Calculator

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参考文献 for DOPE

参考文献是支持产品生物活性的出版物。

Mochizuki et al (2013) The role of the helper lipid dioleoylphosphatidylethanolamine (DOPE) for DNA transfection cooperating with a cationic lipid bearing ethylenediamine. Biochim.Biophys.Acta. 1828 412 PMID: 23092705

Kim et al (2015) DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim.Biophys.Acta. 1848 1996 PMID: 26112463

If you know of a relevant reference for DOPE, please let us know.

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关键词: DOPE, DOPE supplier, non-viral, lipoplex, liposome, transfection, reagents, efficicency, enhancers, enhances, Biochemicals, and, Molecular, Biology, Lipid, Nanoparticles, 7175, Tocris Bioscience

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