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FAI

Cat. No. 8011 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： Reagent for RNA SHAPE-MaP experiments in live cells
化学名： 1H-Imidazol-1-yl(2-methyl-3-furanyl)methanone
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for FAI

FAI is a reagent for RNA SHAPE-MaP experiments in live cells. Can be used to map RNA structures in vivo by forming stable 2'-OH adducts with RNA, blocking reverse transcriptase elongation. In vitro, FAI activity can be quenched with Dithiothreitol (Cat. No. 3154) and can be used to probe the secondary structure of mouse embryonic stem cell 5S rRNA. At elevated temperatures, FAI forms more stable adducts and exhibits less hydrolysis than NAI (Cat. No 7004).

技术数据 for FAI

分子量 176.17
公式 C9H8N2O2
储存 Store at -20°C
CAS Number 1415238-77-5
PubChem ID 66563653
InChI Key OOBPIWAAJBRELM-UHFFFAOYSA-N
Smiles O=C(C=1C=COC1C)N2C=NC=C2

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for FAI

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 17.62 100

制备储备液 for FAI

以下数据基于产品分子量 176.17。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 5.68 mL 28.38 mL 56.76 mL
5 mM 1.14 mL 5.68 mL 11.35 mL
10 mM 0.57 mL 2.84 mL 5.68 mL
50 mM 0.11 mL 0.57 mL 1.14 mL

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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参考文献 for FAI

参考文献是支持产品生物活性的出版物。

Chan et al (2017) Comparative analysis reveals furoyl in vivo selective hydroxyl acylation analyzed by primer extension reagents form stable ribosyl ester adducts. Biochemistry 56 18111 PMID: 28319368

Spitale et al (2013) RNA SHAPE analysis in living cells. Nat.Chem.Biol. 9 18 PMID: 23178934

If you know of a relevant reference for FAI, please let us know.

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关键词: FAI, FAI supplier, reagent, shape, map, RNA, adduct, live, cells, rRNA, stable, stability, less, hydrolysis, block, reverse, transcriptase, DNA,, and, Protein, Synthesis, Biochemicals, Molecular, Biology, 8011, Tocris Bioscience

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Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。

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