FNIR-Tag, NHS ester

Cat. No. 7373 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Near-infrared (NIR) fluorescent dye (Reactive group: NHS ester) optimized for in vivo imaging

化学名： 2-((E)-2-((E)-2-(3-((4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxobutyl)dimethylammonio)propoxy)-3-(2-((E)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-5-sulfonatoindolin-2-ylidene)ethylidene)cyclohex-1-en-1-yl)vinyl)-1-(2-(2-(2-methoxyethoxy)ethoxy)ethyl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate

纯度： ≥70% (HPLC)

实验方案 (1)

文献 (1)

生物活性 Scientific Data 技术数据溶解性计算器说明书参考文献

生物活性 for FNIR-Tag, NHS ester

Key information: FNIR-Tag, NHS ester is a near-infrared fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines with a high degree of labeling (DOL).

Application: Designed for in vivo imaging. Also suitable for flow cytometry.

Properties and Photophysical Data: FNIR-Tag, NHS ester provides a higher degree of labeling (DOL) with less aggregation than a leading competitor product when used for antibody conjugation. FNIR-Tag, NHS ester- antibody conjugates exhibit reduced liver uptake in vivo compared with leading competitor conjugates. In addition, FNIR-Tag, NHS ester conjugates are significantly brighter than leading competitor conjugates at similar labeling densities in vivo and in vitro. Antibody conjugation efficiency is greatest at pH 7.4. Excitation and emission maxima (λ) are 766 nM and 788 nM, respectively; quantum yield = 0.099; extinction coefficient = 200,000 M^-1cm^-1; A280 correction factor = 0.05.

For further information about how FNIR-Tag, NHS ester may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Scientific Data

Flow Cytometry

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Application of FNIR-Tag, NHS ester in vitro. C166 mouse endothelial cells were incubated with 20 nM virus-like particles (VLPs) at 37 °C for 1 h. Samples were extensively washed to remove VLPs not bound to the cellular surface and subsequently fixed to preserve any interactions prior to analysis by flow cytometry. FNIR-Tag conjugates (red) were significantly brighter than IR-800CW conjugates (black) at similar labeling at low (A) and high (B) fluorophore loadings. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512

In vivo Imaging

in vivo fluorescent images of FNIR-Tag, NHS and competitor in mouse post-injection

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Application of FNIR-Tag, NHS ester in vivo. (A) Representative images demonstrating detection of virus-like particle (VLP) conjugates in the liver at 2 h (left panel) and 24 h (right panel) post injection. Scale bar represents epifluorescent in total radiant efficiency. (B) Quantitative analysis of the fluorescence intensity detected at 2 and 24 h post injection. Data are mean value ± standard error. Statistical analysis was performed between groups at the same time point using Student’s t-test. *p ≤ 0.05; **p ≤ 0.01. For more information on this work read Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512

许可信息

Sold under license from the National Institute of Health

Optical Data for FNIR-Tag, NHS ester

Emission Color	Near-IR
Brightness
λ_abs	765 nm
λ_em	788 nm
Extinction Coefficient (ε)	200,000 M^-1cm^-1
Quantum Yield (φ)	0.099
Closest Laser line	750 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Correction Factor 280	0.05
Application	Flow Cytometry, In Vivo Imaging

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

技术数据 for FNIR-Tag, NHS ester

分子量	1157.4
公式	C₅₇H₈₀N₄O₁₇S₂
储存	Store at -20°C
纯度	≥70% (HPLC)
CAS Number	2365033-54-9
InChI Key	UZSWYHGTBPSLCH-UHFFFAOYSA-N
Smiles	[O-]S(=O)(C1=CC=C2[N+](CCOCCOCCOC)=C(C(C)(C2=C1)C)/C=C/C3=C(/C(CCC3)=C/C=C4C(C)(C5=CC(S([O-])(=O)=O)=CC=C5N\4CCOCCOCCOC)C)OCCC[N+](CCCC(ON6C(CCC6=O)=O)=O)(C)C)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for FNIR-Tag, NHS ester

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
	DMSO	11.57	10
	DMF	11.57	10

制备储备液 for FNIR-Tag, NHS ester

以下数据基于产品分子量 1157.4。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.1 mM	8.64 mL	43.2 mL	86.4 mL
0.5 mM	1.73 mL	8.64 mL	17.28 mL
1 mM	0.86 mL	4.32 mL	8.64 mL
5 mM	0.17 mL	0.86 mL	1.73 mL

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产品说明书 for FNIR-Tag, NHS ester

分析证书/产品说明书

化学品安全技术说明书

参考文献 for FNIR-Tag, NHS ester

参考文献是支持产品生物活性的出版物。

Luciano et al (2019) A nonaggregating heptamethine cyanine for building brighter labeled biomolecules. ACS Chem.Biol. 14 934 PMID: 31030512

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关键词: FNIR-Tag, NHS ester, FNIR-Tag, NHS ester supplier, near, infrared, fluorescent, dye, fluorescence, imaging, flow, cytometry, amine, labeling, labelling, NHS, ester, Cyanine, Dyes, (Cy, Dyes), Near, Infrared, (NIR), Fluorescent, 7373, Tocris Bioscience

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