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Submit ReviewJanelia Fluor® 646, Tetrazine
说明： Red dye (Reactive group: tetrazine). Application: flow cytometry, confocal microscopy, super-resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging
别名： JF646
化学名： 4-((4-(1,2,4,5-Tetrazin-3-yl)benzyl)carbamoyl)-2-(3-(azetidin-1-ium-1-ylidene)-7-(azetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
纯度： ≥90% (HPLC)
生物活性 for Janelia Fluor® 646, Tetrazine
Key Information: Janelia Fluor® 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live cell imaging.
Application: Suitable for flow cytometry, confocal microscopy, super-resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Can be multiplexed for two-color imaging with Janelia Fluor;® 549 Tetrazine (Cat. No. 6502). Cell-permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 646 nm and 664 nm, respectively; quantum yield = 0.54; extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor = 0.19; lactone-zwitterion equilibrium constant (KL-Z) = 0.0012.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 646, Tetrazine
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 646 nm |
| λem | 664 nm |
| Extinction Coefficient (ε) | 152,000 M-1cm-1 |
| Quantum Yield (φ) | 0.54 |
| Closest Laser line | 647 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.19 |
| Application | Confocal Microscopy, SRM, dSTORM, STED, Flow Cytometry |
| Alternative For | Alexa Fluor® 647, Atto 647, Atto 647N, CF640R, CF647, Cyanine 5 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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技术数据 for Janelia Fluor® 646, Tetrazine
| 分子量 | 665.83 |
| 公式 | C38H35N7O3Si |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 2042192-00-5 |
| InChI Key | CPWVRCGDSYQFSV-UHFFFAOYSA-N |
| Smiles | C[Si](C)(C1=C/2)C3=CC(N4CCC4)=CC=C3C(C5=CC(C(NCC6=CC=C(C=C6)C7=NN=CN=N7)=O)=CC=C5C([O-])=O)=C1C=CC2=[N+]8CCC/8 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 646, Tetrazine
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 13.32 | 20 |
制备储备液 for Janelia Fluor® 646, Tetrazine
以下数据基于产品分子量 665.83。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.51 mL | 37.55 mL | 75.09 mL |
| 1 mM | 1.5 mL | 7.51 mL | 15.02 mL |
| 2 mM | 0.75 mL | 3.75 mL | 7.51 mL |
| 10 mM | 0.15 mL | 0.75 mL | 1.5 mL |
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产品说明书 for Janelia Fluor® 646, Tetrazine
参考文献 for Janelia Fluor® 646, Tetrazine
参考文献是支持产品生物活性的出版物。
Zheng et al (2019) Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 5 1602 PMID: 31572787
If you know of a relevant reference for Janelia Fluor® 646, Tetrazine, please let us know.
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关键词: Janelia Fluor 646, Tetrazine, Janelia Fluor 646, Tetrazine supplier, JF646, Janelia, Fluor, 646-SE, fluorescent, dyes, self-labeling, tag, systems, microscopy, cell, permeable, super, resolution, STED, flow, cytometry, SRM, Click, Reactive, Dyes, Click-Reactive, Fluorescent, 7279, Tocris Bioscience
1 篇 Janelia Fluor® 646, Tetrazine 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Janelia Fluor® 646, Tetrazine 的部分引用包括：
Yu et al (2023) Visualizing the disordered nuclear transport machinery in situ. Nature 617 162 PMID: 37100914
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Fluorescent Dyes and Probes Research Product Guide
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