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MTT

Cat. No. 5224 5 篇引用文献 2 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Reagent used in the measurement of cell proliferation
化学名： 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
纯度： ≥98%
说明书
引用文献 (5)
评论 (2)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for MTT

MTT is a reagent used in the measurement of cell proliferation.

技术数据 for MTT

分子量 414.32
公式 C18H16N5SBr
储存 Store at +4°C
纯度 ≥98%
CAS Number 298-93-1
PubChem ID 64965
InChI Key AZKSAVLVSZKNRD-UHFFFAOYSA-M
Smiles CC1=C(C)SC([N+]2=NC(C4=CC=CC=C4)=NN2C3=CC=CC=C3)=N1.[Br-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for MTT

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
PBS 5 13

制备储备液 for MTT

以下数据基于产品分子量 414.32。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.13 mM 18.57 mL 92.83 mL 185.66 mL
0.65 mM 3.71 mL 18.57 mL 37.13 mL
1.3 mM 1.86 mL 9.28 mL 18.57 mL
6.5 mM 0.37 mL 1.86 mL 3.71 mL

Molarity Calculator

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Reconstitution Calculator

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Dilution Calculator

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产品说明书 for MTT

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for MTT

参考文献是支持产品生物活性的出版物。

If you know of a relevant reference for MTT, please let us know.

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关键词: MTT, MTT supplier, MTT, measurement, cell, proliferation, Biochemicals, and, Molecular, Biology, 5224, Tocris Bioscience

5 篇 MTT 的引用文献

引用文献是使用了 Tocris 产品的出版物。 MTT 的部分引用包括：

Yusuke et al (2021) Osteoblasts derived from mouse mandible enhance tumor growth of prostate cancer more than osteoblasts derived from long bone. J Bone Oncol 26 100346 PMID: 33425674

Evan et al (2022) Targeted Degradation of 53BP1 Using Ubiquitin Variant Induced Proximity. Biomolecules 12 PMID: 35454069

Xiao et al (2020) Analysis of Key Genes Regulating the Warburg Effect in Patients with Gastrointestinal Cancers and Selective Inhibition of This Metabolic Pathway in Liver Cancer Cells. Onco Targets Ther 13 7295-7304 PMID: 32801756

Jeffrey L et al (2023) Aldose reductase inhibition decelerates optic nerve degeneration by alleviating retinal microglia activation. Sci Rep 13 5592 PMID: 37019993

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MTT 的评论

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Detection of apoptosis via cell proliferation assay MTT was carried out.
By Anonymous on 02/11/2019
种属： Human

Detection of apoptosis via cell proliferation assay MTT was carried out.The 10 µl solution of MTT is added to one of the 96 well- plate and then incubate the plate for 4 hours. After incubation time the medium from wells was discarded and 100 µl of acid-propanol added to all well of the plate and incubated for 30 minutes at room temperature. Then the absorbance of each well were measured at 340 nm by plate reader.

review image
MTT as a tool to estimate cell proliferation.
By Anonymous on 12/12/2018
分析类型： In Vitro
种属： Mouse
细胞系/组织： Cardiomyocytes

HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol). Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.

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