O-Propargyl-puromycin

Cat. No. 7391 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Puromycin analog for labeling newly synthesized proteins

化学名： 3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine

纯度： ≥97% (HPLC)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for O-Propargyl-puromycin

O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.

Optical Data for O-Propargyl-puromycin

λ_em	275 nm
Reactivity	Click
Application	Fluorescence Microscopy, Flow Cytometry

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for O-Propargyl-puromycin

分子量	495.54
公式	C₂₄H₂₉N₇O₅
储存	Store at -20°C
纯度	≥97% (HPLC)
CAS Number	1416561-90-4
PubChem ID	71576433
InChI Key	JXBIGWQNNSJLQK-IYRMOJGWSA-N
Smiles	O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for O-Propargyl-puromycin

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	49.55	100

制备储备液 for O-Propargyl-puromycin

以下数据基于产品分子量 495.54。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	2.02 mL	10.09 mL	20.18 mL
5 mM	0.4 mL	2.02 mL	4.04 mL
10 mM	0.2 mL	1.01 mL	2.02 mL
50 mM	0.04 mL	0.2 mL	0.4 mL

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产品说明书 for O-Propargyl-puromycin

分析证书/产品说明书

化学品安全技术说明书

参考文献 for O-Propargyl-puromycin

参考文献是支持产品生物活性的出版物。

Liu et al (2012) Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 109 413 PMID: 22160674

Morral et al (2020) Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 26 845 PMID: 32396863

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