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Submit ReviewOcean Blue, SE
说明： Blue fluorescent dye (Reactive group: NHS ester). Application: flow cytometry, fluorescence microscopy
化学名： 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester
纯度： ≥95% (HPLC)
生物活性 for Ocean Blue, SE
Key information: Ocean Blue, SE is a blue fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines.
Application: Flow cytometry, fluorescence microscopy.
Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M-1cm-1; A280 correction factor = 0.15.
Please see the protocol for further information on protein/antibody labeling and conjugation.
Note: This product is typically prepared in DMSO.
Optical Data for Ocean Blue, SE
| Emission Color | Blue |
|---|---|
| Brightness | |
| λabs | 405 nm |
| λem | 455 nm |
| Extinction Coefficient (ε) | 36000 M-1cm-1 |
| Quantum Yield (φ) | 0.78 |
| Closest Laser line | 405 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.15 |
| Application | Flow Cytometry, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Ocean Blue, SE
| 分子量 | 339.2 |
| 公式 | C14H7F2NO7 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 215868-33-0 |
| PubChem ID | 56927770 |
| InChI Key | NZYXABPVYJRICY-UHFFFAOYSA-N |
| Smiles | OC1=C(C=C2C=C(C(OC2=C1F)=O)C(ON3C(CCC3=O)=O)=O)F |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Ocean Blue, SE
参考文献 for Ocean Blue, SE
参考文献是支持产品生物活性的出版物。
Sun et al (1998) Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorg.Med.Chem.Lett. 8 3107 PMID: 9873685
Brandtzaeg et al (1998) The increasing power of immunohistochemistry and immunocytochemistry. J.Immunol.Methods 216 49 PMID: 9760215
Haugland et al (2001) Antibody conjugates for cell biology. Curr.Protoc.Cell Biol. PMID: 18228336
Cox et al (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. Biotechniques 36 114 PMID: 14740493
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关键词: Ocean Blue, SE, Ocean Blue, SE supplier, Blue, fluorescent, dye, Standard, Fluorescein,, Coumarin, and, Rhodamine, Dyes, Flow, Cytometry, 6489, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals